Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7281
Title: Clinical value of anti-live Leishmania (Viannia) braziliensis immunoglobulin G subclasses, detected by flow cytometry, for diagnosing active localized cutaneous leishmaniasis
Authors: Rocha, Roberta Dias Rodrigues
Gontijo, Célia Maria Ferreira
Santos, Silvana Maria Elói
Carvalho, Andréa Teixeira de
Oliveira, Rodrigo Corrêa de
Ferrari, Teresa Cristina de Abreu
Marques, Marcos José
Mayrink, Wilson
Martins Filho, Olindo Assis
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Leishmanioses. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazil/Universidade Federal de Ouro Preto. Escola de Farmacia. Ouro Preto, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil
Universidade Federal de Juiz de Fora. Laboratorio de Imunologia. Juiz de Fora, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazil
Abstract: OBJECTIVE: To evaluate the clinical value of flow cytometry anti-live promastigate antibody (FC-ALPA), for diagnosing active cutaneous leishmaniasis. METHOD: Serum samples from 145 individuals living in endemic areas for localized cutaneous leishmaniasis (population 1) were classified as having the disease or not and then tested for their IgG reactivity by indirect immunofluorescence assay and FC-ALPA-IgG. The results of FC-ALPA-IgG were expressed as percentage of positive fluorescent parasite. Both tests were also evaluated in serum samples of people with visceral leishmaniasis and Chagas disease (population 1A). RESULTS: In population 1, FC-ALPA-IgG performed better than the immunofluorescence assay regarding sensitivity, specificity and predictive values. Analysis of the results according to the likelihood ratios indicated that a percentage of positive fluorescent parasite <or=60 practically excludes the diagnosis of localized cutaneous leishmaniasis (likelihood ratio=0.07), whereas at >60% it reinforces diagnosis of the disease (likelihood ratio = 7.0). Immunofluorescent assay is of little value (likelihood ratio=2.04). In population 1A, both tests performed worse, but FC-ALPA-IgG achieved better statistical indexes than immunofluorescent assay. CONCLUSION: The FC-ALPA-IgG is a valuable method for serological diagnosis of localized cutaneous leishmaniasis. FC-ALPA-IgG1/ALPA-IgG2 combined analysis is an additional serological tool for discriminating localized visceral leishmaniasis, Chagas disease and visceral leishmaniasis in areas where these infections co-exist.
Keywords: localized cutaneous leishmaniasis
anti-live Leishmania (V.)
braziliensis promastigotes
IgG antibodies
serodiagnosis
flow cytometry
performance indexes
Issue Date: 2013
Publisher: Blackwell Scientific Publications
Citation: 101- ROCHA, Roberta Dias Rodrigues et al. Clinical value of anti-live Leishmania (Viannia) braziliensis immunoglobulin G subclasses, detected by flow cytometry, for diagnosing active localized cutaneous leishmaniasis. Trop Med Int Health. 2006 Feb;11(2):156-66.
ISSN: 1360-2276
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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