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dc.contributor.authorSilva, Robson Amaro Augusto da
dc.contributor.authorTavares, Natalia Machado
dc.contributor.authorCosta, Dirceu Joaquim
dc.contributor.authorPitombo, Maiana A
dc.contributor.authorBarbosa, Larissa
dc.contributor.authorFukutani, Kiyoshi Ferreira
dc.contributor.authorMiranda, José Carlos
dc.contributor.authorOliveira, Camila Indiani de
dc.contributor.authorValenzuela, Jesus G
dc.contributor.authorBarral, Aldina Maria Prado
dc.contributor.authorSoto, Manuel
dc.contributor.authorBarral Netto, Manoel
dc.contributor.authorBrodskyn, Claudia Ida
dc.date.accessioned2014-02-10T14:08:38Z
dc.date.available2014-02-10T14:08:38Z
dc.date.issued2011
dc.identifier.citationSILVA, R. R. A. da et al. DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis. Acta Tropica, v. 120, n. 3, p. 185-190, 2011.
dc.identifier.issn0001-706X
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/7306
dc.language.isoeng
dc.publisherElsevier
dc.rightsopen access
dc.titleDNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis
dc.typeArticle
dc.identifier.doi10.1016/j.actatropica.2011.08.007
dc.description.abstractenIt was recently shown that immunization of hamsters with DNA plasmids coding LJM19, a sand fly salivary protein, partially protected against a challenge with Leishmania chagasi, whereas immunization with KMP11 DNA plasmid, a Leishmania antigen, induced protection against L. donovani infection. In the present study, we evaluated the protective effect of immunization with both LJM19 and KMP11 DNA plasmid together. Concerning the protection against an infection by L. chagasi, immunization with DNA plasmids coding LJM19 or KMP11, as well as with both plasmids combined, induced IFN-γ production in draining lymph nodes at 7, 14 and 21 days post-immunization. Immunized hamsters challenged with L. chagasi plus Salivary Gland Sonicate (SGS) from Lutzomyia longipalpis showed an enhancement of IFN-γ/IL-10 and IFN-γ/TGF-ß in draining lymph nodes after 7 and 14 days of infection. Two and five months after challenge, immunized animals showed reduced parasite load in the liver and spleen, as well as increased IFN-γ/IL-10 and IFN-γ/TGF-ß ratios in the spleen. Furthermore, immunized animals remained with a normal hematological profile even five months after the challenge, whereas L. chagasi in unimmunized hamsters lead to a significant anemia. The protection observed with LJM19 or KMP11 DNA plasmids used alone was very similar to the protection obtained by the combination of both plasmids
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto Multidisciplinar em Saúde. Vitória da Conquista, BA, Brasil / Faculdade de Medicina. Instituto de Ciências da Saúde. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. United States of America
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationUniversidad Autónoma de Madrid. Departamento de Biologia Molecular. Centro de Biologia Molecular Severo Ochoa. Madrid, Spain
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationInstituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. (iii-INCT). São Paulo, SP, Brasil
dc.subject.enHamster
dc.subject.enLeishmania chagasi
dc.subject.enVisceral leishmaniasis
dc.subject.enSaliva
dc.subject.enDNA plasmids
dc.subject.enProtection
dc.subject.decsProteínas de Insetos/imunologia
dc.subject.decsLeishmaniose Visceral/prevenção & controle
dc.subject.decsGlicoproteínas de Membrana/imunologia
dc.subject.decsProteínas de Protozoários/imunologia
dc.subject.decsPsychodidae/imunologia
dc.subject.decsProteínas e Peptídeos Salivares/imunologia
dc.subject.decsVacinas de DNA/imunologia
dc.subject.decsAnimais
dc.subject.decsCricetinae
dc.subject.decsFeminino
dc.subject.decsProteínas de Insetos/genética
dc.subject.decsInterferon gama/secreção
dc.subject.decsInterleucina-10/secreção
dc.subject.decsLeishmaniose Visceral/imunologia
dc.subject.decsFígado/parasitologia
dc.subject.decsLinfonodos/imunologia
dc.subject.decsMasculino
dc.subject.decsGlicoproteínas de Membrana/genética
dc.subject.decsMesocricetus
dc.subject.decsProteínas Recombinantes/administração & dosagem
dc.subject.decsBaço/imunologia
dc.subject.decsBaço/parasitologia
dc.subject.decsFator de Crescimento Transformador beta/secreção
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