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dc.contributor.authorPrates, Deboraci Brito
dc.contributor.authorSantos, Théo de Araújo
dc.contributor.authorLuz, Nívea Farias
dc.contributor.authorAndrade, Bruno de Bezerril
dc.contributor.authorCosta, Jaqueline França
dc.contributor.authorAfonso, Lilian Maria
dc.contributor.authorClarêncio, Jorge
dc.contributor.authorMiranda, José Carlos
dc.contributor.authorBozza, Patrícia Torres
dc.contributor.authorReis, George A. dos
dc.contributor.authorBrodskyn, Claudia Ida
dc.contributor.authorBarral Netto, Manoel
dc.contributor.authorBorges, Valeria de Matos
dc.contributor.authorBarral, Aldina Maria Prado
dc.date.accessioned2014-02-17T19:36:00Z
dc.date.available2014-02-17T19:36:00Z
dc.date.issued2011
dc.identifier.citationPRATES, D. B. et al. Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils. Journal of Leukocyte Biology, v. 90, n. 3, p.575-582, 2011.
dc.identifier.issn1938-3673
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/7316
dc.language.isoeng
dc.publisherSociety for Leukocyte Biology
dc.rightsopen access
dc.titleLutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils
dc.typeArticle
dc.identifier.doi10.1189/jlb.0211105
dc.description.abstractenNeutrophils are considered the host's first line of defense against infections and have been implicated in the immunopathogenesis of Leishmaniasis. Leishmania parasites are inoculated alongside vectors' saliva, which is a rich source of pharmacologically active substances that interfere with host immune response. In the present study, we tested the hypothesis that salivary components from Lutzomyia longipalpis, an important vector of visceral Leishmaniasis, enhance neutrophil apoptosis. Murine inflammatory peritoneal neutrophils cultured in the presence of SGS presented increased surface expression of FasL and underwent caspase-dependent and FasL-mediated apoptosis. This proapoptosis effect of SGS on neutrophils was abrogated by pretreatment with protease as well as preincubation with antisaliva antibodies. Furthermore, in the presence of Leishmania chagasi, SGS also increased apoptosis on neutrophils and increased PGE(2) release and decreased ROS production by neutrophils, while enhancing parasite viability inside these cells. The increased parasite burden was abrogated by treatment with z-VAD, a pan caspase inhibitor, and NS-398, a COX-2 inhibitor. In the presence of SGS, Leishmania-infected neutrophils produced higher levels of MCP-1 and attracted a high number of macrophages by chemotaxis in vitro assays. Both of these events were abrogated by pretreatment of neutrophils with bindarit, an inhibitor of CCL2/MCP-1 expression. Taken together, our data support the hypothesis that vector salivary proteins trigger caspase-dependent and FasL-mediated apoptosis, thereby favoring Leishmania survival inside neutrophils, which may represent an important mechanism for the establishment of Leishmania infection.
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
dc.creator.affilliationNational Institute of Allergy and Infectious Diseases. National Institutes of Health. Laboratory of Parasitic Diseases. Besthesda, MD, USA
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationInstituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, Brasil
dc.subject.decsApoptose
dc.subject.decsLeishmaniose/imunologia
dc.subject.decsNeutrófilos/patologia
dc.subject.decsNeutrófilos/parasitologia
dc.subject.decsPsychodidae/imunologia
dc.subject.decsSaliva/imunologia
dc.subject.decsAnimais
dc.subject.decsCaspases/metabolismo
dc.subject.decsQuimiotaxia
dc.subject.decsQuimiocina CCL2/metabolismo
dc.subject.decsFeminino
dc.subject.decsProteína Ligante Fas/metabolismo
dc.subject.decsInterações Hospedeiro-Parasita
dc.subject.decsImmunoblotting
dc.subject.decsLeishmania
dc.subject.decsLeishmaniose/parasitologia
dc.subject.decsMacrófagos/imunologia
dc.subject.decsMasculino
dc.subject.decsCamundongos
dc.subject.decsCamundongos Endogâmicos C57BL
dc.subject.decsNeutrófilos/imunologia
dc.subject.decsPsychodidae/parasitologia
dc.subject.decsEspécies de Oxigênio Reativas/metabolismo
dc.subject.decsSaliva/química
dc.subject.decsSaliva/parasitologia
dc.subject.decsGlândulas Salivares/citologia
dc.subject.decsGlândulas Salivares/imunologia
dc.subject.decsGlândulas Salivares/parasitologia
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