Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7318
Title: Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp
Authors: Guimarães, Elisalva Teixeira
Cruz, Gabriela da Silva
Jesus, Alan Araújo de
Carvalho, Acácia Fernandes Lacerda de
Rogatto, Silvia Regina
Pereira, Lygia da Veiga
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Laboratório de Genética Humana e Mutagênese. Departamento de Biologia Geral. Salvador, BA, Brasil
Universidade de São Paulo. Faculdade de Medicina. Departamento de Urologia. Botucatu, SP, Brasil / Centro de Pesquisas Hospital A.C. Camargo. São Paulo, SP, Brasil
Universidade de São Paulo. Instituto de Biociências. Departamento de Biologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Abstract: Objective: Several studies have demonstrated that human dental pulp is a source of mesenchymal stem cells. To better understand the biological properties of these cells we isolated and characterized stem cells from the dental pulp of EGFP transgenic mice. Methods: The pulp tissue was gently separated from the roots of teeth extracted from C57BL/ 6 mice, and cultured under appropriate conditions. Flow cytometry, RT-PCR, light microscopy (staining for alkaline phosphatase) and immunofluorescence were used to investigate the expression of stem cell markers. The presence of chromosomal abnormalities was evaluated by G banding. Results: The mouse dental pulp stem cells (mDPSC) were highly proliferative, plasticadherent, and exhibited a polymorphic morphology predominantly with stellate or fusiform shapes. The presence of cell clusters was observed in cultures of mDPSC. Some cells were positive for alkaline phosphatase. The karyotype was normal until the 5th passage. The Pou5f1/Oct-4 and ZFP42/Rex-1, but not Nanog transcripts were detected in mDPSC. Flow cytometry and fluorescence analyses revealed the presence of a heterogeneous population positive for embryonic and mesenchymal cell markers. Adipogenic, chondrogenic and osteogenic differentiation was achieved after two weeks of cell culture under chemically defined in vitro conditions. In addition, some elongated cells spontaneously acquired a contraction capacity. Conclusions: Our results reinforce that the dental pulp is an important source of adult stem cells and encourage studies on therapeutic potential of mDPSC in experimental disease models
Keywords: Dental pulp
Stem cells
Mouse
Phenotype
Differentiation
DeCS: Células-Tronco Adultas/citologia
Polpa Dentária/citologia
Polpa Dentária/embriologia
Células-Tronco Embrionárias/citologia
Células Mesenquimais Estromais/citologia
Adipogenia
Animais
Diferenciação Celular
Condrogênese
Proteínas de Ligação a DNA/biossíntese
Proteínas de Ligação a DNA/genética
Citometria de Fluxo
Marcadores Genéticos
Cariótipo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Fator 3 de Transcrição de Octâmero/biossíntese
Fator 3 de Transcrição de Octâmero/genética
Osteogênese
Fenótipo
Fatores de Transcrição/biossíntese
Fatores de Transcrição/genética
Issue Date: 2011
Publisher: Elsevier
Citation: GUIMARÃES, E. T. et al. Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp. Archives of Oral Biology, v. 56, n. 11, p. 1247-1255, 2011.
DOI: 10.1016/j.archoralbio.2011.05.008
ISSN: 1879-1506
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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