Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7350
Title: Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis
Authors: Barbosa, Artur F
Sangiorgi, Bruno B
Galdino, Suely L
Barral Netto, Manoel
Pitta, Ivan R
Pinheiro, Antonio Luiz Barbosa
Affilliation: Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil / University of Pernambuco. Laboratory Planning and Synthesis of Drugs. Recife, PE, Brasil / São Camilo School. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Pernambuco. Laboratory Planning and Synthesis of Drugs. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Pernambuco. Laboratory Planning and Synthesis of Drugs. Recife, PE, Brasil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brasil / University of São Paulo. National Institute of Optics and Photonics. São Paulo, SP, Brasil
Abstract: Cutaneous and mucocutaneous leishmaniasis are diseases characterized by skin or mucosal manifestations. In the new world, Leishmania braziliensis is the main etiological agent of cutaneous leishmaniasis, condition that may evolve to the mucocutaneous form. The therapeutic arsenal routinely employed to treat infected patients is unsatisfactory, especially for pentavalent antimonials, treatment recommended by the WHO, as they are often highly toxic, poorly tolerated and of variable effectiveness. This work aimed to evaluate in vitro the effectiveness of photodynamic antimicrobial chemotherapy as a new approach for the treatment of leishmaniasis. MATERIALS AND METHODS: A laser (λ = 660 nm, 40 mW, 4.2 J/cm2 , and 8.4 J/cm2 , CW) associated to phenothiazine's derivatives (5 and 10 µg/ml, toluidine blue O, methylene blue, or phenothiazine) on the promastigote forms of L. braziliensis in a single session. Samples were removed and analyzed in a hemocytometer 72 hours after PACT and viability of the parasites was assessed in quadruplicates. RESULTS: An important decrease in the number of viable parasites on all treated groups in comparison to their controls was observed as all tested compounds lead to significant parasite lethality being the highest lethality achieved with 10 µg/ml of TBO. No lethality was observed on groups treated with laser or with any of the compounds separately. CONCLUSIONS: TBO presented higher parasite lethality in comparison to MB with impressive reduction from 1 hour to 5 minutes of pre-incubation time.
Keywords: Laser-therapy
Leishmaniasis
Methylene blue
Toluidine blue O
Treatment
DeCS: Leishmania braziliensis/efeitos de drogas
Leishmaniose Cutânea/quimioterapia
Azul de Metileno/uso terapêutico
Fotoquimioterapia
Fármacos Fotossensibilizantes/uso terapêutico
Cloreto de Tolônio/uso terapêutico
Tripanossomicidas/uso terapêutico
Humanos
Azul de Metileno/farmacologia
Fenotiazinas/farmacologia
Fenotiazinas/uso terapêutico
Fármacos Fotossensibilizantes/farmacologia
Cloreto de Tolônio/farmacologia
Tripanossomicidas/farmacologia
Issue Date: 2012
Publisher: Wiley Periodicals, Inc
Citation: BARBOSA, A. F. et al. Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis. Lasers in Surgery and Medicine, v. 44, n. 10, p. 850-855, 2012.
DOI: 10.1002/lsm.22099
ISSN: 1096-9101
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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