Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7406
Title: Potential immunomodulatory effects of plant lectins in Schistosoma mansoni infection
Authors: Reis, Eliana Almeida Gomes
Athanazio, Daniel Abensur
Cavada, Benildo Sousa
Teixeira, Edson Holanda
Pinto, Vicente de Paulo Teixeira
Carmo, Theomira Mauadie de Azevedo
Reis, Alice
Trocolli, Graziela
Croda, Julio Henrique Rosa
Harn Junior, Donald A
Barral Netto, Manoel
Reis, Mitermayer Galvão dos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal do Ceará. Departamento de Bioquímica e Biologia Molecular. Fortaleza, CE, Brasil
Universidade Federal de Ceará. Faculdade de Medicina. Sobral, CE, Brasil
Universidade Federal de Ceará. Faculdade de Medicina. Sobral, CE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Harvard School of Public Health. Department of Immunology and Infectious Diseases. Boston, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Lectins are sugar-binding glycoproteins that can stimulate, in a non-antigen-specific fashion, lymphocytes, leading to proliferation and cytokine production. Some lectins are utilized as in vitro mitogenic lymphocyte stimulators and their use as immunomodulators against infectious diseases has been evaluated experimentally. In the experimental murine model, the immune response to schistosomiasis is Th1-like during the initial stage of infection, with a shift towards a Th2-like response after oviposition. We report the response of schistosomiasis patients' (n=37) peripheral blood mononuclear cells (PBMC) to stimulation by lectins, including newly isolated lectins from Brazilian flora, and by Schistosomamansoni soluble egg antigens (SEA). Cytokine production upon lectin stimulation ex vivo was assessed in PBMC supernatants, collected at 24 and 72 h, by sandwich ELISA to IL-5, IL-10, TNF-alpha and IFN-gamma. In PBMC from infected patients all but one of the lectins induced a Th2-like cytokine response, characterized by elevated IL-5 production that was higher than that induced by SEA stimulation alone. Our results show that the Th2 environment present during schistosomiasis is not affected and that it may be further stimulated by the presence of lectins.
Keywords: Lectins
Schistosoma mansoni
Cytokines
DeCS: Fatores Imunológicos/farmacologia
Lectinas/farmacologia
Leucócitos Mononucleares/efeitos de drogas
Leucócitos Mononucleares/imunologia
Esquistossomose mansoni/imunologia
Adolescente
Adulto
Animais
Células Cultivadas
Criança
Citocinas/secreção
Ensaio de Imunoadsorção Enzimática
Feminino
Humanos
Masculino
Meia-Idade
Issue Date: 2008
Publisher: Elsevier B.V
Citation: REIS, E. A. G. et al. Potential immunomodulatory effects of plant lectins in Schistosoma mansoni infection. Acta Tropica, v. 108, n. 2/3, p. 160-165, 2008.
DOI: 10.1016/j.actatropica.2008.05.025
ISSN: 1873-6254
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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