Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7502
Title: Monitoring Leptospira strain collections: the need for quality control.
Authors: Cerqueira, Gustavo Maia de
McBride, Alan John Alexander
Queiroz, Adriano
Pinto, Luciano S
Silva, Éverton Fagonde
Hartskeerl, Rudy A
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Dellagostin, Odir Antonio
Affilliation: Federal University of Pelotas. Biotechnology Centre, Pelotas, RS, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital das Clínicas. Salvador, BA, Brasil
Federal University of Pelotas. Biotechnology Centre, Pelotas, RS, Brasil
Federal University of Pelotas. Biotechnology Centre, Pelotas, RS, Brasil
Department of Biomedical Research. Royal Tropical Institute. Amsterdam, The Netherlands
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Division of Infectious Diseases Weill Medical College of Cornell University. New York, NY
Federal University of Pelotas. Biotechnology Centre, Pelotas, RS, Brasil
Abstract: The purpose of this study was to perform a 16S sequence-based quality control of two Leptospira strain collections. 16S rRNA gene sequencing was used to verify two Leptospira reference collections provided by the World Health Organization and maintained at a reference laboratory for leptospirosis in Brazil. Among the 89 serovars evaluated, four conflicting strains were identified in one of the collections. Although 16S rRNA gene sequencing cannot identify Leptospira beyond the species level, it is suitable for the identification of contamination and quality control of leptospiral reference collections. This study highlights the importance of the availability of high-quality 16S rRNA sequences in public databases. In addition, it emphasizes the need for periodical verifications and quality control of Leptospira reference collections
DeCS: Leptospira/isolamento & purificação
Animais
Sequência de Bases
Primers do DNA
Leptospira/genética
RNA Ribossômico 16S/genética
Especificidade da Espécie
Issue Date: 2010
Publisher: American Society of Tropical Medicine and Hygiene
Citation: CERQUEIRA, G. M. et al. Monitoring Leptospira strain collections: the need for quality control. American Journal of Tropical Medicine and Hygiene, v. 82, n. 1, p. 83-87, 2010.
DOI: 10.4269/ajtmh.2010.09-0558
ISSN: 1476-1645
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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