Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7521
Full metadata record
DC FieldValue
dc.contributor.authorBlank, Walter A
dc.contributor.authorReis, Eliana Almeida Gomes
dc.contributor.authorThiong'o, Fred W
dc.contributor.authorBraghiroli, João F
dc.contributor.authorSantos, Jarbas M
dc.contributor.authorMelo, Paulo Roberto Santana de
dc.contributor.authorGuimarães, Isabel C. S
dc.contributor.authorSilva, Luciano Kalabric
dc.contributor.authorCarmo, Theomira Mauadie de Azevedo
dc.contributor.authorReis, Mitermayer Galvão dos
dc.contributor.authorBlanton, Ronald Edward
dc.date.accessioned2014-04-14T18:58:05Z
dc.date.available2014-04-14T18:58:05Z
dc.date.issued2009
dc.identifier.citationBLANK, W. A. et al. Analysis of Schistosoma mansoni population structure using total fecal egg sampling. Journal of Parasitolofy, v. 95, n. 4, p. 881-889, 2009.
dc.identifier.issn1937-2345
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/7521
dc.language.isoeng
dc.publisherNational Institute of Health
dc.rightsopen access
dc.titleAnalysis of Schistosoma mansoni population structure using total fecal egg sampling.
dc.typeArticle
dc.description.abstractenMany parasite populations are difficult to sample because they are not uniformly distributed between several host species and are often not easily collected from the living host, thereby limiting sample size and possibly distorting the representation of the population. For the parasite Schistosoma mansoni, we investigated the use of eggs, in aggregate, from the stools of infected individuals as a simple and representative sample. Previously, we demonstrated that microsatellite allele frequencies can be accurately estimated from pooled DNA of cloned S. mansoni adults. Here, we show that genotyping of parasite populations from reproductively isolated laboratory strains can be used to identify these specific populations based on characteristic patterns of allele frequencies, as observed by polyacrylamide gel electrophoresis and automated sequencer analysis of fluorescently labeled PCR products. Microsatellites used to genotype aggregates of eggs collected from stools of infected individuals produced results consistent with the geographic distribution of the samples. Preferential amplification of smaller alleles, and stutter PCR products, had negligible effect on measurement of genetic differentiation. Direct analysis of total stool eggs can be an important approach to questions of population genetics for this parasite by increasing the sample size to thousands per infected individual and by reducing bias.
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationUniversity of Nairobi. Nairobi, Kenya / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFederal University of Bahia. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationCenter for Global Health and Diseases. Case Western Reserve University. Cleveland, Ohio
dc.subject.decsDNA de Helmintos/química
dc.subject.decsFezes/parasitologia
dc.subject.decsRepetições de Microssatélites/genética
dc.subject.decsSchistosoma mansoni/isolamento & purificação
dc.subject.decsEsquistossomose mansoni/parasitologia
dc.subject.decsEletroforese em Gel de Poliacrilamida
dc.subject.decsAnimais
dc.subject.decsBrasil
dc.subject.decsFeminino
dc.subject.decsFrequência do Gene
dc.subject.decsGenótipo
dc.subject.decsHumanos
dc.subject.decsQuênia
dc.subject.decsMasculino
dc.subject.decsÓvulo
dc.subject.decsReação em Cadeia da Polimerase
dc.subject.decsSchistosoma mansoni/classificação
dc.subject.decsSchistosoma mansoni/genética
dc.subject.decsAnálise de Sequência
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Blank W A Direct analysis of .....pdf602.43 kBAdobe PDFThumbnail
View/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.