Author | Oliveira, Susana Carla Pires Sampaio | |
Author | Monteiro, Juliana Santos de Carvalho | |
Author | Santos, Gustavo Miranda Pires | |
Author | Sampaio, Fernando José Pires | |
Author | Gomes Júnior, Rafael Araújo | |
Author | Gesteira, Maria de Fatima Malvar | |
Author | Brugnera Junior, Aldo | |
Author | Zanin, Fátima A. A | |
Author | Santos, Marcos André Vannier dos | |
Author | Pinheiro, Antonio Luiz Barbosa | |
Access date | 2014-05-09T17:04:24Z | |
Available date | 2014-05-09T17:04:24Z | |
Document date | 2014 | |
Citation | De OLIVEIRA, S. C. P. S. et al. In vitro influence of photodynamic antimicrobial chemotherapy on staphylococcus aureus by using phenothiazines derivatives associated with laser/LED Light. Progress in Biomedical Optics and Imaging Proceedings of SPIE, v. 8932, p.89320H1-H7 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/7596 | |
Language | eng | pt_BR |
Publisher | SPIE | pt_BR |
Rights | open access | pt_BR |
Title | In vitro influence of photodynamic antimicrobial chemotherapy on staphylococcus aureus by using phenothiazines derivatives associated with laser/LED Light | pt_BR |
Type | Article | pt_BR |
DOI | 10.1117/12.2038576 | |
Abstract | The aim of this study was to evaluate the effect of photodynamic antimicrobial chemotherapy (PACT) using
phenothiazinium dyes - PTZ irradiated with red laser (ʎ660nm) or red-orange LED (ʎ632±2nm) on Staphylococcus
aureus in vitro. triplicate tests were performed in 10 groups: control, Laser (L1+P- and L2+P-) bacterial suspensions were
irradiated only with laser energy 2.4 and 4.8 J/cm2 respectively, (Led1+P- and Led2+P-) irradiated only with LED energy
2.4 and 4.8 J/cm2 respectively, (L1+P+ and L2+P+) irradiated with laser in the presence of 1μg/ml of photosensitizer,
(Led1+P+ and Led2+P+) irradiated with LED in the presence of 1μg/ml of photosensitizer and finally (L-P+) only in the
presence of PTZ dye. Bactericidal effect of the PACT was assessed by counting colony-forming units. The results
showed no significant difference on regards different energy densities on group PACT for both lights. PACT groups
(L2+P+ and Led2+P+) compared to the Control showed significant reduction of CFUs. LED/Laser groups (L2+P- and
Led2+P-) compared to control and PTZ groups showed also significant differences as groups LED/Laser (4.8J/cm2)
increased the average of CFUs. Although the results of this study have shown a reduction in average number of colonyforming
units by the appropriate Laser or LED-dye treatment combination, it this topic requires further investigation. | pt_BR |
Affilliation | Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Estate University of Feira de Santana. Department of Biology. Feira de Santana, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitéria. Salvador, BA, Brasil / School of Medicine. Universidade Federal da Bahia. Salvador, BA, Brasil / School of Medicine and Public Health. FDC. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitéria. Salvador, BA, Brasil / School of Medicine. Universidade Federal da Bahia. Salvador, BA, Brasil / School of Medicine and Public Health. FDC. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitéria. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil | pt_BR |
Subject | Photodynamic antimicrobial chemotherapy | pt_BR |
Subject | Staphylococcus aureus | pt_BR |
Subject | Light emitting-diodes | pt_BR |