Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7649
Title: Plasma lipoproteins in visceral leishmaniasis and their effect on Leishmania-infected macrophages
Authors: Soares, Neci Matos
Leal, T. F
Fiúza, M. C
Reis, Eliana Almeida Gomes
Souza, M. A. L
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
Affilliation: Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de An álises Clínicas e Toxicológicas. Salvador, BA, Brasil
Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de An álises Clínicas e Toxicológicas. Salvador, BA, Brasil /Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de An álises Clínicas e Toxicológicas. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Abstract: This work aimed at investigating the lipid profile of zoonotic visceral leishmaniasis (VL) patients' sera and the effect of lipoproteins on the in vitro production of tumour necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, IL-10 and IL-12 by Leishmania infantum-infected and uninfected macrophages. Lipids were quantified in 26 VL patients' sera and 26 healthy controls from a VL endemic area. The patients' sera had higher triglyceride and very low density lipoprotein (VLDL) levels, and much lower apolipoprotein A1, total cholesterol, low density lipoprotein (LDL) and high density lipoprotein (HDL) levels than the control sera. Lipoprotein fractions were obtained by ultracentrifugation of sera. The addition of LDL and HDL to Leishmania-infected and uninfected macrophages, in physiological concentrations, enhanced the production of IL-6 and IL-10, but not of IL-12. LDL stimulated the production of TNF-alpha only in infected macrophages, whereas HDL stimulated the production of lower amounts of TNF-alpha in both infected and uninfected macrophages. VLDL stimulated only the production of IL-10. It is proposed herein that LDL may influence the development of VL by promoting the production of TNF-alpha by infected macrophages. A decrease in plasma LDL in some VL patients (to 20 mg/mL or less); however, would tend to reduce the production of TNF-alpha and therefore to limit the development of immune-mediated pathology, not withstanding the fact that it would perhaps increase the permissiveness of macrophages to Leishmania growth.
Keywords: Cytokines
Leishmania
Lipoproteins
DeCS: Leishmaniose Visceral/imunologia
Leishmaniose Visceral/patologia
Lipídeos/sangue
Lipoproteínas/sangue
Macrófagos/imunologia
Macrófagos/parasitologia
Adulto
Animais
Criança
Citocinas/secreção
Pré-Escolar
Feminino
Humanos
Lactente
Lipoproteínas/isolamento & purificação
Masculino
Ultracentrifugação
Adulto Jovem
Issue Date: 2010
Publisher: Blackwell Publishing Ltd
Citation: SOARES, N. M. et al. Plasma lipoproteins in visceral leishmaniasis and their effect on Leishmania-infected macrophages. Parasite Immunology, v. 32, p.259-266, 2010.
DOI: 10.1111/j.1365-3024.2009.01187.x
ISSN: 1365-3024
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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