Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7867
Title: Comparative study of the expression of cellular cycle proteins in cervical intraepithelial lesions
Authors: Queiroz, Conceição
Silva, Tânia Maria Correia
Alves, Venâncio Avancini Ferreira
Villa, Luisa L.
Costa, Maria Cecília
Travassos, Ana Gabriela Álvares
Araújo Filho, José Bouzas
Studart, Eduardo
Cheto, Tatiana
Freitas, Luiz Antonio Rodrigues de
Affilliation: Federal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
São Paulo Federal University. Department of Pathology. Adolfo Lutz Institute. São Paulo, SP, Brasil
Ludwig Institute for Cancer Research. Virology Department. São Paulo, SP, Brasil
Ludwig Institute for Cancer Research. Virology Department. São Paulo, SP, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics. Department of Pathology, Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Interaction of human papilloma virus oncoproteins E6 and E7 with cell cycle proteins leads to disturbances of the cell cycle mechanism and subsequent alteration in the expression of some proteins, such as p16INK4a, cyclin D1, p53 and KI67. In this study, we compared alterations in the expression of these proteins during several stages of intraepitelial cervical carcinogenesis. Accordingly, an immunohistochemical study was performed on 50 cervical biopsies, including negative cases and intraepithelial neoplasias. The expression patterns of these markers were correlated with the histopathological diagnosis and infection with HPV. The p16INK4a, followed by Ki67, showed better correlation with cancer progression than p53 and cyclin D1, which recommends their use in the evaluation of cervical carcinogenesis. These monoclonal antibodies can be applied to cervical biopsy specimens to identify lesions transformed by oncogenic HPV, separating CIN 1 (p16INK4a positive) and identifying high-grade lesions by an increase in the cellular proliferation index (Ki67). In this way, we propose immunomarkers that can be applied in clinical practice to separate patients who need a conservative therapeutic approach from those who require a more aggressive treatment.
Keywords: Cervical neoplasia
P16INK4a
Cyclin D1
Ki67
p53
DeCS: Proteínas de Ciclo Celular/metabolismo
Neoplasia Intraepitelial Cervical/metabolismo
Neoplasias do Colo do Útero/metabolismo
Adulto
Idoso
Idoso de 80 Anos ou mais
Biópsia
Proliferação de Células
Neoplasia Intraepitelial Cervical/patologia
Colo do Útero/anatomia & histologia
Colo do Útero/metabolismo
Ciclina D1/metabolismo
Inibidor p16 de Quinase Ciclina-Dependente/metabolismo
Feminino
Humanos
Antígeno Ki-67/metabolismo
Meia-Idade
Marcadores Biológicos de Tumor/metabolismo
Neoplasias do Colo do Útero/patologia
Issue Date: 2006
Publisher: Elsevier
Citation: QUEIROZ, C. et al. P16(INK4a) expression as a potential prognostic marker in cervical pre-neoplastic and neoplastic lesions. Pathology Research Practice, v. 202, n. 2, p. 731-737, 2006.
DOI: 10.1016/j.prp.2006.07.003
ISSN: 0344-0338
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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