Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8155
Title: Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi.
Authors: Araujo, Adriano Fernando da Silva
Oliveira, Gabriel de
Vasconcelos, Juliana Fraga
Ersching, Jonatan
Dominguez, Mariana Ribeiro
Vasconcelos, José Ronnie Carvalho de
Machado, Alexandre Vieira
Gazzinelli, Ricardo Tostes
Romero, Oscar Bruna
Soares, Milena Botelho Pereira
Rodrigues, Maurício Martins
Affilliation: Escola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Biologia Celular. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Escola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Escola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Escola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil / Departamento de Biociências. Instituto de Saúde e Sociedade - UNIFESP. Santos, SP, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, BH, Brasil / University of Massachusetts Medical School. Division of Infectious Disease and Immunology. Department of Medicine. Worcester, MA, USA
Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Florianopolis, SC, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
Escola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Abstract: In earlier studies, we reported that a heterologous prime-boost regimen using recombinant plasmid DNA followed by replicationdefective adenovirus vector, both containing Trypanosoma cruzi genes encoding trans-sialidase (TS) and amastigote surface protein (ASP) 2, provided protective immunity against experimental infection with a reticulotropic strain of this human protozoan parasite. Herein, we tested the outcome of genetic vaccination of F1 (CB10XBALB/c) mice challenged withmyotropic parasite strains (Brazil and Colombian). Initially, we determined that the coadministration during priming of a DNA plasmid containing themurine IL-12 gene improved the immune response and was essential for protective immunity elicited by the heterologous prime-boost regimen in susceptiblemale mice against acute lethal infections with these parasites. The prophylactic or therapeutic vaccination of resistant female mice led to a drastic reduction in the number of inflammatory infiltrates in cardiac and skeletal muscles during the chronic phase of infection with either strain. Analysis of the electrocardiographic parameters showed that prophylactic vaccination reduced the frequencies of sinus arrhythmia and atrioventricular block. Our results confirmed that prophylactic vaccination using the TS and ASP-2 genes benefits the host against acute and chronic pathologies caused by T. cruzi and should be further evaluated for the development of a veterinary or human vaccine against Chagas disease.
keywords: Trypanosoma cruzi
Doença de Chagas
Vacinação
Animais
Camundongos
Patologia
Issue Date: 2014
Publisher: Hindawi Publishing Corporation
Citation: ARAÚJO, Adriano Fernando da Silva et al. Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi. Mediators of Inflammation, 2014, p. 605023, 2014.
DOI: 10.1155/2014/605023
ISSN: 1466-1861
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
BA - IGM - Artigos de Periódicos

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