Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8184
Title: The effect of apoptotic cell recognition on macrophage polarization and mycobacterial persistence
Authors: Fulco, Tatiana de Oliveira
Andrade, Priscila Ribeiro
Mattos Barbosa, Mayara Garcia de
Pinto, Thiago Gomes Toledo
Ferreira, Paula Fernandez
Ferreira, Helen
Nery, José Augusto da Costa
Corte-Real, Suzana
Borges, Valeria de Matos
Moraes, Milton Ozório
Sarno, Euzenir Nunes
Sampaio, Elizabeth Pereira
Pinheiro, Roberta Olmo
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Abstract: Intracellular Mycobacterium leprae infection modifies host macrophage programming, creating a protective niche for bacterial survival. The milieu regulating cellular apoptosis in the tissue plays an important role in defining susceptible and/or resistant phenotypes. A higher density of apoptotic cells has been demonstrated in paucibacillary leprosy lesions than in multibacillary ones. However, the effect of apoptotic cell removal on M. leprae-stimulated cells has yet to be fully elucidated. In this study, we investigate whether apoptotic cell removal (efferocytosis) induces different phenotypes in pro- (Mo1) and anti- (Mo2) inflammatory macrophages in the presence of M. leprae. We stimulated Mo1 and Mo2 cells with M. leprae in the presence or absence of apoptotic cells and subsequently evaluated the M. leprae uptake, cell phenotype, and cytokine pattern in the supernatants. In the presence of M. leprae and apoptotic cells, Mo1 macrophages changed their phenotype towards Mo2, displaying increased CD163 and SRA-I expression as well as higher phagocytic capacity. Efferocytosis increased M. leprae survival in Mo1 cells, accompanied by reduced IL-15 and IL-6 and increased TGF-β and IL-10 secretion. Mo1 cells primed with M. leprae in the presence of apoptotic cells induced the secretion of Th2 cytokines IL-4 and IL-13 in autologous T cells when compared with cultures stimulated with M. leprae or apoptotic cells alone. Efferocytosis did not alter the Mo2 cell phenotype or cytokine secretion profile, except for TGF-B. Based on these data, we suggest that, in paucibacillary leprosy patients, efferocytosis contributes to mycobacterial persistence by increasing the Mo2 population and sustaining the infection.
keywords: Mycobacterium leprae
Apoptose
Hanseníase paucibacilar
Bactérias
Issue Date: 2014
Publisher: American Society for Microbiology
Citation: FULCO, Tatiana de Oliveira et al. The effect of apoptotic cell recognition on macrophage polarization and mycobacterial persistence. Infection and Immunity, p. 1-31, jul 2014.
DOI: 10.1128/IAI.02194-14
ISSN: 0019-9567
Copyright: open access
Revised document: Não
Publication status: Publicado
Appears in Collections:IOC - Preprint
BA - IGM - Preprint

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