Author | Pinho, Rosa Teixeira de | |
Author | Dutra, Helio dos Santos | |
Author | De Simone, Salvatore Giovanni | |
Author | Pontes-de-Carvalho, Lain Carlos | |
Access date | 2014-09-10T14:17:25Z | |
Available date | 2014-09-10T14:17:25Z | |
Document date | 1991 | |
Citation | PINHO, Rosa Teixeira de et al. A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide. Acta Tropica, v. 50, n. 1, p. 29-38, 1991. | pt_BR |
ISSN | 0001-706X | |
URI | https://www.arca.fiocruz.br/handle/icict/8342 | |
Language | eng | pt_BR |
Publisher | Elsevier Science Publishers B.V | pt_BR |
Rights | open access | |
Title | A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide | pt_BR |
Type | Article | |
Abstract | Glass wool, hydrophilic cotton wool, non-electrically charged BIO-GEL P2 and common tissue paper
columns were used to purify trypomastigotes from a mixed Trypanosoma cruzi population grown in axenic
culture medium. With all these columns, highly purified (up to 98%) trypomastigote preparations were
obtained. Trypomastigote yields from cotton wool, BIO-GEL P2 and common tissue paper columns were
not as high as from glass wool columns, from which yields varied from 69 to 80%. Purification on glass
wool did not affect trypomastigote infectivity or virulence. Dead trypomastigotes could not be purified on
glass wool columns. A glass-adherent amphiphilic peptide of 45 kDa, present in the cell membrane, was
isolated from epimastigote but not from trypomastigote preparations. | pt_BR |
Affilliation | WHO Collaborating Center on Immunology of Parasitic Diseases. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | WHO Collaborating Center on Immunology of Parasitic Diseases. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Biologia Celular e Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niteroi, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Glass wool purification | pt_BR |
Subject | Trypomastigotes | pt_BR |
Subject | Surface peptide | pt_BR |
Subject | Triton X-114 | pt_BR |
DeCS | Doença de Chagas/parasitologia | pt_BR |
DeCS | Vidro | pt_BR |
DeCS | Proteínas de Membrana/análise | pt_BR |
DeCS | Proteínas de Protozoários/análise | pt_BR |
DeCS | Trypanosoma cruzi/isolamento & purificação | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Cromatografia DEAE-Celulose | pt_BR |
DeCS | Eletroforese em Gel de Poliacrilamida | pt_BR |
DeCS | Insetos Vetores/parasitologia | pt_BR |
DeCS | Masculino | pt_BR |
DeCS | Proteínas de Membrana/metabolismo | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Proteínas de Protozoários/metabolismo | pt_BR |
DeCS | Triatoma/parasitologia | pt_BR |
DeCS | Trypanosoma cruzi/química | pt_BR |