Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8554
Title: Evidence for the involvement of descending pain-inhibitory mechanisms in the antinociceptive effect of hecogenin acetate.
Authors: Gama, Kelly Barbosa
Quintans, Jullyana de Souza Siqueira
Antoniolli, Angelo Roberto
Quintans Junior, Lucindo José
Santana, Wagno Alcântara
Branco, Alexsandro
Soares, Milena Botelho Pereira
Villarreal, Cristiane Flora
Affilliation: Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil
Universidade Federal de Sergipe. Departamento de Fisiologia. Aracaju, SE, Brasil
Universidade Federal de Sergipe. Departamento de Fisiologia. Aracaju, SE, Brasil
Universidade Federal de Sergipe. Departamento de Fisiologia. Aracaju, SE, Brasil
Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil
Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Hecogenin is a sapogenin present in the leaves of species from the Agave genus, with a wide spectrum of reported pharmacological activities. The present study was undertaken to evaluate whether hecogenin acetate (1) has antinociceptive properties and to determine its mechanism of action. The nociceptive threshold was evaluated using the tail flick test in mice. Mice motor performance was evaluated in a Rotarod test. By using Fos expression as a marker of neural activation, the involvement of the periaqueductal gray in 1-induced antinociception was evaluated. Intraperitoneal administration of 1 (5−40 mg/kg) produced a dose-dependent increase in the tail flick latency time compared to vehicle-treated group (p < 0.01). Mice treated with 1 (40 mg/kg) did not show motor performance alterations. The antinociception of 1 (40 mg/kg) was prevented by naloxone (nonselective opioid receptor antagonist; 5 mg/kg), CTOP (μ-opioid receptor antagonist; 1 mg/kg), nor-BNI (κ- opioid receptor antagonist; 0.5 mg/kg), naltrindole (δ-opioid receptor antagonist; 3 mg/kg), or glibenclamide (ATP-sensitive K+ channel blocker; 2 mg/kg). Systemic administration of 1 (5−40 mg/kg) increased the number of Fos positive cells in the periaqueductal gray. The present study has demonstrated for the first time that 1 produces consistent antinociception mediated by opioid receptors and endogenous analgesic mechanisms
DeCS: Agave/química
Analgésicos/farmacologia
Dor/quimioterapia
Compostos de Espiro/farmacologia
Esteroides/farmacologia
Animais
Glibureto/farmacologia
Canais KATP/antagonistas & inibidores
Masculino
Camundongos
Estrutura Molecular
Naltrexona/farmacologia
Substância Cinzenta Periaquedutal/efeitos de drogas
Folhas de Planta/química
Receptores Opioides kappa/antagonistas & inibidores
Issue Date: 2013
Publisher: ACS Publications
Citation: GAMA, K. B. et al. Evidence for the involvement of descending pain-inhibitory mechanisms in the antinociceptive effect of hecogenin acetate. Journal of Natural Products, v. 76, n. 4, p. 559-563, 2013.
DOI: 10.1021/np3007342
ISSN: 1520-6025
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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