Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8643
Title: Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails.
Authors: Couto, Flávia Fernanda Bubula
Coelho, Paulo Marcos Zech
Pereira, Neusa Araújo
Kusel, John Robert
Katz, Naftale
Passos, Liana konovaloff Jannotti
Mattos, Ana Carolina Alves de
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
University of Glasgow Faculty of Biomedical and Life Sciences. Division of Infection and Immunity. Scotland, UK
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Moluscário Lobato Paraense. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
Abstract: To elucidate the mechanisms of antischistosoma resistance, drug-resistant Schistosoma mansoni laboratory isolates are essential. We developed a new method for inducing resistance to praziquantel (PZQ) using successive drug treatments of Biomphalaria glabrata snails infected with S. mansoni. Infected B. glabrata were treated three times with 100 mg/kg PZQ for five consecutive days with a one-week interval between them. After the treatment, the cercariae (LE-PZQ) produced from these snails and the LE strains (susceptible) were used to infect mice. Forty-five days after infection, mice were treated with 200, 400 or 800 mg/kg PZQ. Thirty days post-treatment, we observed that the mean number of worms recovered by perfusion was significantly higher in the group of mice infected with the LE-PZQ isolate treated with 200 and 400 mg/kg in comparison to the LE strain with the same treatment. Moreover, there was a significant difference between the ED50 (effective dose required to kill 50% of the worms) of the LE-PZQ isolate (362 mg/kg) and the LE strain (68 mg/kg). In the in vitro assays, the worms of the LE-PZQ isolate were also less susceptible to PZQ. Thus, the use of infected snails as an experimental model for development of resistance to S. mansoni is effective, fast, simple and cheap.
Keywords: Schistosoma mansoni
Biomphalaria glabrata
praziquantel
resistance induction/new method
Issue Date: 2011
Publisher: Fundação Oswaldo Cruz
Citation: COUTO, Flávia Fernanda Bubula et al. Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biompharia glabrata snails. Memorias do Instituto Oswaldo Cruz, 106(2): 153-157, 2011.
ISSN: 0074-0276
http://dx.doi.org/10.1590/S0074-02762011000200006
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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