Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8683
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dc.contributor.authorFranco, Priscila Silva
dc.contributor.authorGomes, Angelica de Oliveira
dc.contributor.authorBarbosa, Bellisa de Freitas
dc.contributor.authorAngeloni, Mariana Bodini
dc.contributor.authorSilva, Neide Maria
dc.contributor.authorCarvalho, Andréa Teixeira
dc.contributor.authorMartins Filho, Olindo Assis
dc.contributor.authorSilva, Daise Aparecida Oliveira
dc.contributor.authorMineo, José Roberto
dc.date.accessioned2014-10-28T12:49:06Z
dc.date.available2014-10-28T12:49:06Z
dc.date.issued2011
dc.identifier.citationFRANCO, Priscila Silva et at. Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection. Placenta, 32(11): 838-844, 2011.
dc.identifier.issn0143-4004
dc.identifier.issn10.1016/j.placenta.2011.08.012
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/8683
dc.language.isoeng
dc.publisherElsevier Ltd.
dc.rightsopen access
dc.titleAzithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection.
dc.typeArticle
dc.description.abstractenToxoplasma gondii is an important pathogen which may cause fetal infection if primary infection. Our previous studies have used human choriocarcinoma trophoblastic cells (BeWo cell line) as experimental model of T. gondii infection involving placental microenvironment. This study aimed to examine the effects of azithromycin and spiramycin against T. gondiiinfection in BeWo cells. Cells were treated with different concentrations of the macrolide antibiotics and analyzed first for cell viability using thiazolyl blue tetrazole (MTT) assay. As cell viability was significantly decreased with drug concentrations higher than 400 μg/mL, the concentration range used in further experiments was from 50 to 400 μg/mL. The number of infected cells and intracellular replication of T. gondii decreased after treatment with each drug. The infection induced up-regulation of the macrophage migration inhibitory factor (MIF), which was also enhanced in infected cells after treatment with azithromycin, but not with spiramycin. Analysis of the cytokine profile showed increase TNF-α, IL-10 and IL-4 production, but decreased IFN-γ levels, were detected in infected cells and treated with each drug. In conclusion, treatment of human trophoblastic BeWo cells with with azithromycin or spiramycin is able to control the infection and replication of T. gondii. In addition, treatment with these macrolides, especially with azityromycin induces an anti-inflammatory response and high MIF production, which can be important for the establishment and maintenance of a viable pregnancy during T. gondii infection.
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunopatologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunopatologia. Uberlândia, MG, Brazil
dc.subject.enAzithromycin
dc.subject.enSpiramycin
dc.subject.enBeWo cells
dc.subject.enCytokines
dc.subject.enToxoplasma gondii
Appears in Collections:MG - IRR - Artigos de Periódicos



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