Plasmodium berghei NK65 induces cerebral leukocyte recruitment in vivo: An intravital microscopic study.

Universidade Federal de Minas Gerais Departamento de Parasitologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Núcleo de Neurociências. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Núcleo de Neurociências. Belo Horizonte, MG, Brazil
Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Escola de Farmácia. Departamento de Análises Clínicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Laboratório de Imunofarmacologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil. Minas Gerais, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil. Minas Gerais, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil. Minas Gerais, Brazil
Universidade Federal de Minas Gerais Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Núcleo de Neurociências. Belo Horizonte, MG, Brazil

Abstract

Malaria is second only to tuberculosis as the leading cause of morbidity and mortality as a consequence of a single infectious agent. Much of the pathology of malaria arises from the inappropriate or excessive immune response mounted by the host in an attempt to eliminate the parasite. We here report the inflammatory changes observed in the cerebral microvasculature of C57BL/6 and BALB/c mice that had been inoculated with Plasmodium berghei NK65, a lethal strain of rodent malaria. Although no neurological signs were observed in experimentally infected mice, inflammation of the cerebral microvasculature was clearly evident. Histopathological analysis demonstrated that alterations in cerebral tissue were more intense in infected C57Bl/6 mice than in infected BALB/c animals. Intravital microscopic examination of the cerebral microvasculature revealed increased leukocyte rolling and adhesion in pial venules of infected mice compared with non-infected animals. The extravasation of Evans blue dye into the cerebral parenchyma was also elevated in infected mice in comparison with their non-infected counterparts. Additionally, protein levels of TNF-α, MIG/CXCL9, MCP-1/CCL2, MIP-1α/CCL3 and RANTES/CCL5 were up-regulated in brain samples derived from infected C57Bl/6 mice. Taken together, the data reported here illustrate the complex strain-dependent relationships between leukocyte recruitment, blood brain barrier permeability and chemokine production.

Keywords

Experimental malaria
Plasmodium berghei
NK65
Intravital microscopy
Cerebral inflammation
Chemokines
Microcirculation

Publisher

Elsevier Ltda

Citation

QUEIROZ, Norinne Lacerda et al. Plasmodium berghei NK65 induces cerebral leukocyte recruitment in vivo: An intravital microscopic study. Acta Tropica, volume 120, fascisculo 1–2, p.31–39, out-nov. 2011.

ISSN

0001-706X
10.1016/j.actatropica.2011.04.020

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8771

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Open access

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