Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8839
Title: Molecular epidemiology of endemic human T-lymphotropic virus type 1 in a rural community in Guinea-Bissau.
Authors: van Tienen, Carla
Silva, Thushan I. de
Alcantara, Luiz Carlos Júnior
Onyango, Clayton O
Jarju, Sheikh
Gonçalves, Nato
Vincent, Tim
Aaby, Peter
Whittle, Hilton
van der Loeff, Maarten Schim
Cotten, Matthew
Affilliation: Virology Medical Research Council. Fajara, The Gambia / Medical Microbiology & Infectious Diseases. Erasmus Medical Centre. Rotterdam, The Netherlands
Virology Medical Research Council. Fajara, The Gambia / University College London. UCL Centre for Medical Molecular Virology. Division of Infection and Immunity. London, United Kingdom
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado em Saúde Pública. Salvador, BA, Brasil / Bahia School of Medicine and Public Health Foundation for Science Development. Salvador, BA, Brasil
Virology Medical Research Council. Fajara, The Gambia / KEMRI-Wellcome Trust Programme. Kilifi, Kenya
Virology Medical Research Council. Fajara, The Gambia
Virology Medical Research Council. Fajara, The Gambia / Projecto de Saúde de Bandim. Bissau, Guinea-Bissau
Virology Medical Research Council. Fajara, The Gambia
Indepth Network. Bissau, Guinea-Bissau
Virology Medical Research Council. Fajara, The Gambia
Virology Medical Research Council. Fajara, The Gambia /
Wellcome Trust Genome Campus Wellcome Trust Sanger Institute. Hinxton, Cambridge, United Kingdom
Abstract: BACKGROUND: Human T-Lymphotropic Virus Type 1 (HTLV-1) infection causes lethal adult T-cell leukemia (ATL) and severely debilitating HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) in up to 5% of infected adults. HTLV-1 is endemic in parts of Africa and the highest prevalence in West Africa (5%) has been reported in Caio, a rural area in the North-West of Guinea-Bissau. It is not known which HTLV-1 variants are present in this community. Sequence data can provide insights in the molecular epidemiology and help to understand the origin and spread of HTLV-1. OBJECTIVE: To gain insight into the molecular diversity of HTLV-1 in West Africa. METHODS: HTLV-1 infected individuals were identified in community surveys between 1990-2007. The complete Long Terminal Repeat (LTR) and p24 coding region of HTLV-1 was sequenced from infected subjects. Socio-demographic data were obtained from community census and from interviews performed by fieldworkers. Phylogenetic analyses were performed to characterize the relationship between the Caio HTLV-1 and HTLV-1 from other parts of the world. RESULTS: LTR and p24 sequences were obtained from 72 individuals (36 LTR, 24 p24 only and 12 both). Consistent with the low evolutionary change of HTLV-1, many of the sequences from unrelated individuals showed 100% nucleotide identity. Most (45 of 46) of the LTR sequences clustered with the Cosmopolitan HTLV-1 subtype 1a, subgroup D (1aD). LTR and p24 sequences from two subjects were divergent and formed a significant cluster with HTLV-1 subtype 1g, and with the most divergent African Simian T-cell Lymphotropic Virus, Tan90. CONCLUSIONS: The Cosmopolitan HTLV-1 1aD predominates in this rural West African community. However, HTLV-1 subtype 1g is also present. This subtype has not been described before in West Africa and may be more widespread than previously thought. These data are in line with the hypothesis that multiple monkey-to-man zoonotic events are contributing to HTLV-1 diversity.
DeCS: Variação Genética
Infecções por HTLV-I/epidemiologia
Infecções por HTLV-I/virologia
Vírus 1 Linfotrópico T Humano/classificação
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Criança
Pré-Escolar
Análise por Conglomerados
Feminino
Genótipo
Guiné-Bissau/epidemiologia
Vírus 1 Linfotrópico T Humano/isolamento & purificação
Humanos
Lactente
Masculino
Meia-Idade
Epidemiologia Molecular
Dados de Sequência Molecular
Filogenia
Prevalência
RNA Viral/genética
Análise de Sequência de DNA
Adulto Jovem
Issue Date: 2012
Publisher: Public Library of Science
Citation: van TIENEN, C. et al. Molecular epidemiology of endemic human T-lymphotropic virus type 1 in a rural community in Guinea-Bissau. PLoS Neglected Tropical Diseases, v. 6, n. 6, p. e1690, 2012.
DOI: 10.1371/journal.pntd.0001690
ISSN: 1935-2735
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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