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https://www.arca.fiocruz.br/handle/icict/8922
ACTIVATION OF SONIC HEDGEHOG SIGNALING IN ORAL SQUAMOUS CELL CARCINOMAS: A PRELIMINARY STUDY.
Proteínas Hedgehog/fisiologia
Neoplasias Bucais/metabolismo
Adulto
Idoso
Idoso de 80 Anos ou mais
Carcinoma de Células Escamosas/patologia
Perfilação da Expressão Gênica
Feminino
Humanos
Imuno-Histoquímica
Neoplasias Bucais/patologia
Masculino
Meia-Idade
Reação em Cadeia da Polimerase
Transdução de Sinal
Author
Affilliation
Hospital A.C. Camargo. Department of Anatomical Pathology. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
University of São Paulo. Dental School. Department of General Pathology. São Paulo, SP, Brasil
Hospital A.C. Camargo. Department of Anatomical Pathology. São Paulo, SP, Brasil / University of São Paulo. Dental School. Department of General Pathology. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
University of São Paulo. Dental School. Department of General Pathology. São Paulo, SP, Brasil
Hospital A.C. Camargo. Department of Anatomical Pathology. São Paulo, SP, Brasil / University of São Paulo. Dental School. Department of General Pathology. São Paulo, SP, Brasil
Abstract
Sonic hedgehog signaling is important for human development, and aberrant regulation of this
pathway can result in the development of tumors. The aim of this study was to examine the expression
of sonic hedgehog signaling molecules in oral squamous cell carcinoma. By quantitative real-time
polymerase chain reaction, the expression of SHH, SMO, PTCH-1, and GLI-1 was analyzed in 30 oral
squamous cell carcinoma cases and 8 samples of nonneoplastic oral mucosa and associated to clinical
pathologic features. The expression of β-catenin, cyclin D1, Wnt-1, and Egfr was evaluated by
immunohistochemistry in 26 available cases of oral squamous cell carcinoma. Normal oral mucosa from
healthy individuals was negative for all genes that were evaluated. SHH, PTCH-1, SMO, and GLI-1
were not expressed in nonneoplastic oral mucosa, and low levels of GLI-1 were observed in
nonneoplastic oral mucosa that was adjacent to the tumor. All oral squamous cell carcinoma cases
expressed high levels of PTCH-1, SMO, and GLI-1 and were devoid of SHH. The expression of SMO
was associated with clinical stage (P = .022) and a borderline association in cervical lymph node
metastasis (P = .053). PTCH-1 expression showed a strong correlation with SMO (rs = 0.64; P b .001)
and GL-1 (rs = 0.70; P b .001); SMO and GLI-1 also correlated with each other (rs, 0.55; P b .001). All
proteins evaluated were expressed as cyclin D1 (92% of samples), β-catenin (73%), Egfr (46%), or Wnt-
1 (32%). Our data demonstrate that sonic hedgehog signaling is activated in oral squamous cell
carcinoma and suggest that this pathway mediates its tumorigenesis.
DeCS
Carcinoma de Células Escamosas/metabolismoProteínas Hedgehog/fisiologia
Neoplasias Bucais/metabolismo
Adulto
Idoso
Idoso de 80 Anos ou mais
Carcinoma de Células Escamosas/patologia
Perfilação da Expressão Gênica
Feminino
Humanos
Imuno-Histoquímica
Neoplasias Bucais/patologia
Masculino
Meia-Idade
Reação em Cadeia da Polimerase
Transdução de Sinal
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