| Author | Oliveira, Susana Carla Pires Sampaio | |
| Author | Santos, Gustavo Miranda Pires | |
| Author | Monteiro, Juliana Santos de Carvalho | |
| Author | Miranda, Anderson Fontes Suzart | |
| Author | Sampaio, Fernando José Pires | |
| Author | Gesteira, Maria de Fatima Malvar | |
| Author | Zanin, Fátima A. A | |
| Author | Santos, Marcos André Vannier dos | |
| Author | Pinheiro, Antonio Luiz Barbosa | |
| Access date | 2015-03-17T18:35:24Z | |
| Available date | 2015-03-17T18:35:24Z | |
| Document date | 2013 | |
| Citation | OLIVEIRA, S. C. P. D. et al. Photodynamic antimicrobial chemotherapy (PACT) using Phenothiazines Derivatives Associated with the Red Laser against Staphylococcus aureus. Proceedings of SPIE, v. 8569, p. 1-6, 2013. | pt_BR |
| ISSN | 0277-786X | |
| URI | https://www.arca.fiocruz.br/handle/icict/9730 | |
| Language | eng | pt_BR |
| Publisher | Society of Photo-optical Instrumentation Engineers | pt_BR |
| Rights | open access | pt_BR |
| Title | Photodynamic antimicrobial chemotherapy (PACT) using Phenothiazines Derivatives Associated with the Red Laser against Staphylococcus aureus | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1117/12.2005560 | pt_BR |
| Abstract | The objective of this study was to evaluate the bactericidal effect of photodynamic antimicrobial chemotherapy (PACT) using phenothiazinium dye (Toluidine blue O and methylene blue) at a low concentration of 1μg/mL irradiated with the red laser at doses of 2.4 e 4.8 J/cm² on strain of Staphylococcus aureus (ATCC 23529) in vitro. For this research, tests
were performed in triplicate and the samples were distributed into six test groups: (L-P-) Negative control (L1+P-) and (L2+P-) bacterial suspensions were irradiated with laser energy 2.4 and 4.8 J/cm2 respectively in the absence of photosensitizer; (L1+P+) and (L2+P+) bacterial suspensions were irradiated with laser in the presence of 1μg/ml of photosensitizer and finally (L-P+) bacterial suspensions only in the presence of phenothiazinium dye. Therefore, were analyzed the potential bactericidal PACT by counting of colony-forming units and analyzed statistically (ANOVA, Tukey test, p<0.05). The results showed that the negative control group when compared with laser group (L2+P-) it was
observed a statistically significant increase (p<0.01) which L2+P- showed a higher number of CFU, on the other hand when compared to L1+P- no statistically significant difference was found, relation to the groups submitted to PACT, only showed a statistically significant reduction relative to the group irradiated L2+P+ (p<0.01) that showed a decrease in the number of CFU. There was no statistically significant difference between the groups submitted to PDT (L1+P+ and L2+P+). Although the results of this study have shown a reduction in average number of colony forming units by the appropriate laser-dye treatment combination, it needs further investigation. | pt_BR |
| Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil. | pt_BR |
| Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil. | pt_BR |
| Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil / Camilo Castelo Branco University. São José dos Campos. São Paulo, SP, Brasil. | pt_BR |
| Subject | Photodynamic antimicrobial chemotherapy | pt_BR |
| Subject | Staphylococcus aureus | pt_BR |
| Subject | Lasers | pt_BR |
