Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/9993
Title: Humoral and cellular immune responses in BALB/c and C57BL/6 mice immunized with cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens, in acute experimental Trypanosoma cruzi infection.
Authors: Pereira, Valéria Rêgo Alves
Lorena, Virginia Maria Barros de
Nakazawa, Mineo
Luna, Carlos Feitosa
Silva, Edimilson Domingos da
Ferreira, Antonio Gomes Pinto
Krieger, Marco Aurélio
Goldenberg, Samuel
Soares, Milena Botelho Pereira
Coutinho, Eridan de Medeiros
Oliveira, Rodrigo Corrêa
Gomes, Yara de Miranda
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. FarManguinhos. Rio de Janeiro, RJ, Brasil /Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. FarManguinhos. Rio de Janeiro, RJ, Brasil /Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Abstract: In previous studies, cytoplasmic repetitive antigen (CRA) and flagellar repetitive antigen (FRA) proteins induced specific humoral and cellular immune responses in susceptible and resistant mice in the absence of Trypanosoma cruzi infection with a significant induction of the Interferon-gamma (IFN-gamma) production in those animals. In this follow-up paper, the immunostimulatory and protective effects of these proteins were evaluated by immunizing with CRA or FRA antigens, BALB/c and C57BL/6 mice and challenging with a T. cruzi (Y strain). Both proteins induced humoral response with high levels of IgG isotypes as well as cellular immunity with high levels of IFN-gamma when compared to controls. However, the lymphocyte proliferative response was minimal. The survival rate at 30 days post-infection was significant in CRA (60%) or FRA (50%)--immunized BALB/c mice and CRA (83.3%)--immunized C57BL/6 mice. Taken as a whole these findings indicate that CRA and FRA are immunogenic and potentially important for protective immunity.
DeCS: Anticorpos Antiprotozoários/sangue
Antígenos de Protozoários/imunologia
Doença de Chagas/imunologia
Proteínas Recombinantes/imunologia
Trypanosoma cruzi/imunologia
Animais
Antígenos de Protozoários/administração & dosagem
Modelos Animais de Doenças
Ensaio de Imunoadsorção Enzimática
Imunidade Celular
Imunoglobulina G/sangue
Interferon gama/análise
Linfócitos/imunologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Issue Date: 2005
Publisher: Springer Verlag
Citation: PEREIRA, V. R. A. et al. Humoral and cellular immune responses in BALB/c and C57BL/6 mice immunized with cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens, in acute experimental Trypanosoma cruzi infection. Parasitology Research, v. 96, n. 3, p. 154-161, 2005.
DOI: 10.1007/s00436-005-1336-4
ISSN: 0932-0113
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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