Author | Miranda, Gustavo Cabral | |
Author | Jesus, Joilson Ramos de | |
Author | Oliveira, Pablo Rafael Silveira | |
Author | Britto, G. S. G | |
Author | Pontes-de-Carvalho, Lain Carlos | |
Author | Dutra, R. F | |
Author | Neves, Neuza Maria Alcântara | |
Access date | 2015-05-19T17:06:38Z | |
Available date | 2015-05-19T17:06:38Z | |
Document date | 2014 | |
Citation | MIRANDA, G. C. et al. Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors. Journal of Parasitology, v. 100, n. 1, p. 73-78, 2014. | pt_BR |
ISSN | 1937-2345 | |
URI | https://www.arca.fiocruz.br/handle/icict/10434 | |
Language | eng | pt_BR |
Publisher | American Society of Parasitologists | pt_BR |
Rights | open access | pt_BR |
Title | Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors. | pt_BR |
Type | Article | pt_BR |
DOI | dx.doi.org/10.1645/GE-3052.1 | |
Abstract | Diseases such as leishmaniases are important causes of morbidity and mortality in Brazil, and their diagnoses need to be improved. The use of monoclonal antibodies has ensured high specificity to immunodiagnosis. The development of an immunosensor, coupling a monoclonal antibody to a bioelectronic device capable of quickly detecting Leishmania sp. antigens both qualitatively and quantitatively, is a promising alternative for the diagnosis of leishmaniasis due to its high specificity, low cost, and portability, compared with conventional methods. The present work was aimed at developing an immunosensor-based assay for detecting Leishmania infantum antigens in tissues of infected hosts. Four hybridomas producing monoclonal antibodies against L. infantum had their specificity confirmed by enzyme-linked immunosorbent assay. These antibodies were immobilized on a gold surface, covered with a thin film of 2-aminoethanethiol (cysteamine) and glutaraldehyde, blocked with glycine, and placed into contact with extracts of L. infantum -infected and noninfected control hamster spleens. The assay was able to detect 1.8 × 10(4) amastigotes/g of infected tissue. These results demonstrated that this assay may be useful for quantifying L. infantum amastigotes in organs of experimental animals for studies on pathogenesis and immunity and that it is a promising tool for the development of a diagnostic method, based on antigen detection, of human and dog visceral leishmaniasis. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Laboratório de EngenhariaBiomédica. Recife, PE, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil | pt_BR |
DeCS | Anticorpos Monoclonais/uso diagnóstico | pt_BR |
DeCS | Antígenos de Protozoários/isolamento & purificação | pt_BR |
DeCS | Leishmania infantum/imunologia | pt_BR |
DeCS | Leishmaniose Visceral/diagnóstico | pt_BR |
DeCS | Baço/parasitologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Anticorpos Monoclonais/imunologia | pt_BR |
DeCS | Anticorpos Antiprotozoários/uso diagnóstico | pt_BR |
DeCS | Especificidade de Anticorpos | pt_BR |
DeCS | Técnicas Biossensoriais/métodos | pt_BR |
DeCS | Cricetinae | pt_BR |
DeCS | Ensaio de Imunoadsorção Enzimática | pt_BR |
DeCS | Imunoglobulina G/uso diagnóstico | pt_BR |
DeCS | Leishmania infantum/isolamento & purificação | pt_BR |
DeCS | Mesocricetus | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Camundongos Endogâmicos BALB C | pt_BR |
DeCS | Organismos Livres de Patógenos Específicos | pt_BR |
DeCS | Baço/imunologia | pt_BR |