Author | Escobar, Sergio Cuervo | |
Author | Barragán, Monica Losada | |
Author | Pérez, Adriana Umaña | |
Author | Porrozzi, Renato | |
Author | Vahia, Leonardo Saboia | |
Author | Miranda, Luisa Helena Monteiro de | |
Author | Morgado, Fernanda Nazaré | |
Author | Menezes, Rodrigo Caldas | |
Author | Gómez, Myriam Sánchez | |
Author | Cuervo, Patricia | |
Access date | 2015-06-03T17:56:21Z | |
Available date | 2015-06-03T17:56:21Z | |
Document date | 2014 | |
Citation | ESCOBAR, Sergio Cuervo et al. T-cell populations and cytokine expression are impaired in thymus and spleen of protein malnourished BALB/c mice infected with leishmania infantum. Plos One, v.9, n.12, p.e114584, 2014. | pt_BR |
ISSN | 1932-6203 | |
URI | https://www.arca.fiocruz.br/handle/icict/10646 | |
Sponsorship | CNPq, Departamento Administrativo de
Ciencia, Tecnología e Innovación, FIOCRUZ, National University of Colombia Research
Council | pt_BR |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | |
Title | T-cell populations and cytokine expression are impaired in thymus and spleen of protein malnourished BALB/c mice infected with leishmania infantum | pt_BR |
Type | Article | |
DOI | 10.1371/journal.pone.0114584 | |
Abstract | Visceral leishmaniasis (VL) is a parasitic infectious disease that causes significant
morbidity and mortality in the tropical and subtropical regions of the world. Although
infections with visceralizing Leishmania may be asymptomatic, factors such as
undernutrition increase the likelihood of progressing to clinical disease. Protein
malnutrition, the most deleterious cause of malnutrition in developing countries, has
been considered as a primary risk factor for the development of clinical VL.
However, data regarding the immunological basis of this association are scarce.
With the aim to analyze the effects of protein malnutrition on Leishmania infantum
infection, we used BALB/c mice subjected to control or low protein isocaloric diets.
Each animal group was divided into two subgroups and one was infected with L.
infantum resulting in four study groups: animals fed 14% protein diet (CP), animals
fed 4% protein diet (LP), animals fed 14% protein diet and infected (CPi), and
animals fed 4% protein diet and infected (LPi).The susceptibility to L. infantum
infection and immune responses were assessed in terms of body and lymphoid
organ weight, parasite load, lymphocyte subpopulations, and cytokine expression.
LPi mice had a significant reduction of body and lymphoid organ weight and
exhibited a severe decrease of lymphoid follicles in the spleen. Moreover, LPi
animals showed a significant decrease in CD4+CD8+ Tcells in the thymus, whereas
there was an increase of CD4+ and CD8+ T cells percentages in the spleen.
Notably, the cytokine mRNA levels in the thymus and spleen of protein malnourished-infected animals were altered compared to the CP mice. Protein
malnutrition results in a drastic dysregulation of T cells and cytokine expression in
the thymus and spleen of L. infantum-infected BALB/c mice, which may lead to
defective regulation of the thymocyte population and an impaired splenic immune
response, accelerating the events of a normal course of infection. | pt_BR |
Affilliation | Universidad Nacional de Colombia. Laboratorio de Hormonas. Departamento de QuÍmica. Bogotá, Colômbia | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidad Nacional de Colombia. Laboratorio de Hormonas. Departamento de QuÍmica. Bogotá, Colômbia | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidad Nacional de Colombia. Laboratorio de Hormonas. Departamento de QuÍmica. Bogotá, Colômbia | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil | pt_BR |
Subject | Leishmania infantum | pt_BR |
Subject | Leishmaniasis, Visceral | pt_BR |
Subject | Antigens, CD4 | pt_BR |
Subject | Antigens, CD8 | pt_BR |
DeCS | Leishmania infantum | pt_BR |
DeCS | Leishmaniose Visceral | pt_BR |
DeCS | Antígenos CD4 | pt_BR |
DeCS | Antígenos CD8 | pt_BR |
e-ISSN | 1932-6203 | |