Author | Figueira, Cláudio Pereira | |
Author | Carvalhal, Djalma Gomes Ferrão | |
Author | Almeida, Rafaela Andrade | |
Author | Hermida, Micely D' El Rei | |
Author | Touchard, Dominique | |
Author | Robert, Phillipe | |
Author | Pierres, Anne | |
Author | Bongrand, Pierre | |
Author | dosSantos, Washington Luis Conrado | |
Access date | 2015-10-26T14:00:03Z | |
Available date | 2015-10-26T14:00:03Z | |
Document date | 2015 | |
Citation | FIGUEIRA, C. P. et al. Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. Scientific Reports, v. 5, p. 12862, 2015. | pt_BR |
ISSN | 2045-2322 | |
URI | https://www.arca.fiocruz.br/handle/icict/12083 | |
Language | eng | pt_BR |
Publisher | Nature Publishing Group | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmania | pt_BR |
Subject in Portuguese | Fagócitos | pt_BR |
Subject in Portuguese | Monócitos | pt_BR |
Subject in Portuguese | Infecção | pt_BR |
Subject in Portuguese | Fibronectina | pt_BR |
Subject in Portuguese | Parasita | pt_BR |
Title | Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1038/srep12862 | |
Abstract | Contact with Leishmania leads to a decreases in mononuclear phagocyte adherence to connective
tissue. In this work, we studied the early stages of bond formation between VLA4 and fibronectin,
measured the kinetics of membrane alignment and the monocyte cytoplasm spreading area over a
fibronectin-coated surface, and studied the expression of high affinity integrin epitope in uninfected
and Leishmania-infected human monocytes. Our results show that the initial VLA4-mediated
interaction of Leishmania-infected monocyte with a fibronectin-coated surface is preserved, however,
the later stage, leukocyte spreading over the substrate is abrogated in Leishmania-infected cells.
The median of spreading area was 72 [55–89] μm2 for uninfected and 41 [34–51] μm2 for Leishmaniainfected
monocyte. This cytoplasm spread was inhibited using an anti-VLA4 blocking antibody.
After the initial contact with the fibronectrin-coated surface, uninfected monocyte quickly spread
the cytoplasm at a 15 μm2 s−1 ratio whilst Leishmania-infected monocytes only made small contacts
at a 5.5 μm2 s−1 ratio. The expression of high affinity epitope by VLA4 (from 39 ± 21% to 14 ± 3%);
and LFA1 (from 37 ± 32% to 18 ± 16%) molecules was reduced in Leishmania-infected monocytes.
These changes in phagocyte function may be important for parasite dissemination and distribution of
lesions in leishmaniasis. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / 2Universidade do Estado da Bahia. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Aix-Marseille Université. Parc Scientifique de Luminy. Laboratoire Adhésion Cellulaire et Inflammation. Marseille, France | pt_BR |
Affilliation | Aix-Marseille Université. Parc Scientifique de Luminy. Laboratoire Adhésion Cellulaire et Inflammation. Marseille, France | pt_BR |
Affilliation | Aix-Marseille Université. Parc Scientifique de Luminy. Laboratoire Adhésion Cellulaire et Inflammation. Marseille, France | pt_BR |
Affilliation | Aix-Marseille Université. Parc Scientifique de Luminy. Laboratoire Adhésion Cellulaire et Inflammation. Marseille, France | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |