Author | Ferraz, Raquel | |
Author | Cunha, Clarissa Ferreira | |
Author | Pimentel, Maria Inês | |
Author | Lyra, Marcelo Rosandiski | |
Author | Schubach, Armando Oliveira | |
Author | Mendonça, Sérgio Coutinho Furtado de | |
Author | Cruz, Alda Maria da | |
Author | Bertho, Álvaro Luiz | |
Access date | 2015-11-12T15:18:31Z | |
Available date | 2015-11-12T15:18:31Z | |
Document date | 2015 | |
Citation | FERRAZ, Raquel; et al. T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz, v.110, n.5, p.596-605, Aug. 2015. | pt_BR |
ISSN | 0074-0276 | |
URI | https://www.arca.fiocruz.br/handle/icict/12216 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz | pt_BR |
Rights | open access | |
Title | T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil | pt_BR |
Type | Article | |
DOI | 10.1590/0074-02760150039 | |
Abstract | In human cutaneous leishmaniasis (CL), the immune response is mainly mediated by T-cells. The role of CD8+ Tlymphocytes,
which are related to healing or deleterious functions, in affecting clinical outcome is controversial. The
aim of this study was to evaluate T-cell receptor diversity in late-differentiated effector (LDE) and memory CD8+ T-cell
subsets in order to create a profile of specific clones engaged in deleterious or protective CL immune responses. Healthy
subjects, patients with active disease (PAD) and clinically cured patients were enrolled in the study. Total CD8+ T-lymphocytes
showed a disturbance in the expression of the Vβ2, Vβ9, Vβ13.2, Vβ18 and Vβ23 families. The analyses of CD8+
T-lymphocyte subsets showed high frequencies of LDE CD8+ T-lymphocytes expressing Vβ12 and Vβ22 in PAD, as well as
effector-memory CD8+ T-cells expressing Vβ22. We also observed low frequencies of effector and central-memory CD8+
T-cells expressing Vβ2 in PAD, which correlated with a greater lesion size. Particular Vβ expansions point to CD8+ T-cell
clones that are selected during CL immune responses, suggesting that CD8+ T-lymphocytes expressing Vβ12 or Vβ22 are
involved in a LDE response and that Vβ2 contractions in memory CD8+ T-cells are associated with larger lesions. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Núcleo de Purificação Celular (Sorting). Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | undação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | undação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Núcleo de Purificação Celular (Sorting). Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Vβ repertoire | pt_BR |
Subject | Human cutaneous leishmaniasis | pt_BR |
Subject | Flow cytometry | pt_BR |
Subject | CD8+ T-lymphocyte | pt_BR |
Subject | Leishmania braziliensis | pt_BR |
Subject | TCR | pt_BR |
DeCS | Leishmania braziliensis | pt_BR |
DeCS | Citometria de Fluxo | pt_BR |
DeCS | Leishmaniose Cutânea | pt_BR |
e-ISSN | 1678-8060 | |