Author | Cordeiro, Ana Paula | |
Author | Pereira, Rosiane Aparecida Silva | |
Author | Chapeaurouge, Alex | |
Author | Coimbra, Clarice Semião | |
Author | Perales, Jonas | |
Author | Oliveira, Guilherme | |
Author | Candiani, Talitah Michel Sanchez | |
Author | Coimbra, Roney Santos | |
Access date | 2016-03-10T15:07:20Z | |
Available date | 2016-03-10T15:07:20Z | |
Document date | 2015 | |
Citation | CORDEIRO, Ana Paula; et al. Comparative proteomics of cerebrospinal fluid reveals a predictive model for differential diagnosis of pneumococcal, meningococcal, and enteroviral meningitis, and novel putative therapeutic targets. BMC Genomics, v.16, Suppl 5, S11, 2015. | pt_BR |
ISSN | 1471-2164 | |
URI | https://www.arca.fiocruz.br/handle/icict/13107 | |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | |
Title | Comparative proteomics of cerebrospinal fluid reveals a predictive model for differential diagnosis of pneumococcal, meningococcal, and enteroviral meningitis, and novel putative therapeutic targets | pt_BR |
Type | Article | |
DOI | 10.1186/1471-2164-16-S5-S11 | |
Abstract | Background: Meningitis is the inflammation of the meninges in response to infection or chemical agents. While
aseptic meningitis, most frequently caused by enteroviruses, is usually benign with a self-limiting course, bacterial
meningitis remains associated with high morbidity and mortality rates, despite advances in antimicrobial therapy
and intensive care. Fast and accurate differential diagnosis is crucial for assertive choice of the appropriate
therapeutic approach for each form of meningitis.
Methods: We used 2D-PAGE and mass spectrometry to identify the cerebrospinal fluid proteome specifically
related to the host response to pneumococcal, meningococcal, and enteroviral meningitis. The disease-specific
proteome signatures were inspected by pathway analysis.
Results: Unique cerebrospinal fluid proteome signatures were found to the three aetiological forms of meningitis
investigated, and a qualitative predictive model with four protein markers was developed for the differential
diagnosis of these diseases. Nevertheless, pathway analysis of the disease-specific proteomes unveiled that
Kallikrein-kinin system may play a crucial role in the pathophysiological mechanisms leading to brain damage in
bacterial meningitis. Proteins taking part in this cellular process are proposed as putative targets to novel
adjunctive therapies.
Conclusions: Comparative proteomics of cerebrospinal fluid disclosed candidate biomarkers, which were
combined in a qualitative and sequential predictive model with potential to improve the differential diagnosis of
pneumococcal, meningococcal and enteroviral meningitis. Moreover, we present the first evidence of the possible
implication of Kallikrein-kinin system in the pathophysiology of bacterial meningitis. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou (CPqRR). Grupo de Pesquisa em Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou (CPqRR). Grupo de Pesquisa em Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou (CPqRR). Grupo de Pesquisa em Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou (CPqRR). Grupo de Pesquisa em Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | FHEMIG. Hospital de Crianças João Paulo II. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou (CPqRR). Grupo de Pesquisa Informática de Biosistemas. Belo Horizonte, MG, Brasil. | pt_BR |
Subject | Comparative proteomics | pt_BR |
Subject | Cerebrospinal fluid | pt_BR |
Subject | Pneumococcal meningitis | pt_BR |
Subject | Meningococcal meningitis | pt_BR |
Subject | Enteroviral meningitis | pt_BR |
DeCS | Proteômica | pt_BR |
DeCS | Líquido Cefalorraquidiano | pt_BR |
DeCS | Meningite Pneumocócica | pt_BR |
DeCS | Meningite Meningocócica | pt_BR |
DeCS | Meningite viral | pt_BR |