Author | Fukutani, Kiyoshi Ferreira | |
Author | Carvalho, Cristiana Maria Costa Nascimento | |
Author | Gucht, Winke Van der | |
Author | Wollants, Elke | |
Author | Cunha, Antonio Ricardo Khouri | |
Author | Dierckx, Tim | |
Author | Van Ranst, Marc | |
Author | Houspie, Lieselot | |
Author | Bouzas, Maiara Lanna Souza Bacelar | |
Author | Oliveira, Juliana R. | |
Author | Barral, Aldina Maria Prado | |
Author | Van Weyenbergh, Johan Jozef Rosa Maria | |
Author | Oliveira, Camila Indiani de | |
Access date | 2016-04-13T18:32:07Z | |
Available date | 2016-04-13T18:32:07Z | |
Document date | 2015 | |
Citation | FUKUTANIA, Kiyoshi Ferreira et al. Pathogen transcriptional profile in nasopharyngeal aspirates of children with acute respiratory tract infection. Journal of Clinical Virology, v. 69, p. 190-1966, 2015. | pt_BR |
ISSN | 1386-6532 | |
URI | https://www.arca.fiocruz.br/handle/icict/13752 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Title | Pathogen transcriptional profile in nasopharyngeal aspirates of children with acute respiratory tract infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.jcv.2015.06.005 | pt_BR |
Abstract | Background: Acute respiratory tract infections (ARI) present a significant morbidity and pose a global
health burden. Patients are frequently treated with antibiotics although ARI are most commonly caused
by virus, strengthening the need for improved diagnostic methods.
Objectives: Detect viral and bacterial RNA in nasopharyngeal aspirates (NPA) from children aged 6–23
months with ARI using nCounter.
Study design: A custom-designed nCounter probeset containing viral and bacterial targets was tested in
NPA of ARI patients.
Results: Initially, spiked control viral RNAs were detectable in ≥6.25 ng input RNA, indicating absence of
inhibitors in NPA. nCounter applied to a larger NPA sample (n = 61) enabled the multiplex detection of
different pathogens: RNA viruses Parainfluenza virus (PIV 1–3) and RSV A-B in 21%, Human metapneumovirus
(hMPV) in 5%, Bocavirus (BoV), CoV, Influenza virus (IV) A in 3% and, Rhinovirus (RV) in 2% of
samples, respectively. RSV A-B was confirmed by Real Time PCR (86.2–96.9% agreement). DNA virus (AV)
was detected atRNAlevel, reflecting viral replication, in 10% of samples. Bacterial transcripts from Staphylococcus
aureus, Haemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis, Mycoplasma
pneumoniae and Chlamydophila pneumoniae were detected in 77, 69, 26, 8, 3 and 2% of samples, respectively.
Conclusion: nCounter is robust and sensitive for the simultaneous detection of viral (both RNA and DNA)
and bacterial transcripts in NPA with low RNA input (<10 ng). This medium-throughput technique will
increase our understanding of ARI pathogenesis and may provide an evidence-based approach for the
targeted and rational use of antibiotics in pediatric ARI. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil. | pt_BR |
Affilliation | Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pediatrics. Salvador, BA, Brasil. | pt_BR |
Affilliation | Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium. | pt_BR |
Affilliation | Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil. | pt_BR |
Affilliation | Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium. | pt_BR |
Affilliation | Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium. | pt_BR |
Affilliation | Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium. | pt_BR |
Affilliation | Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil. | pt_BR |
Affilliation | Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Department of Pathology. School of Medicine. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Rega Institute for Medical Research. Department of Microbiology and Immunology. KU Leuven, Leuven, Belgium. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia School of Medicine. Post-Graduation Program in Health Sciences. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil. | pt_BR |
Subject | ARI | pt_BR |
Subject | nCounter | pt_BR |
Subject | Diagnostics | pt_BR |
Subject | RSV | pt_BR |