Author | Wunder Junior, Elsio Augusto | |
Author | Figueira, Cláudio Pereira | |
Author | Benaroudj, Nadia | |
Author | Hu, Bo | |
Author | Tong, Brian A | |
Author | Trajtenberg, Felipe | |
Author | Liu, Jun | |
Author | Reis, Mitermayer Galvão dos | |
Author | Charon, Nyles W | |
Author | Buschiazzo, Alejandro | |
Author | Picardeau, Mathieu | |
Author | Ko, Albert Icksang | |
Access date | 2016-08-16T16:39:34Z | |
Available date | 2016-08-16T16:39:34Z | |
Document date | 2016 | |
Citation | WUNDER JÚNIOR, E. A. et al. A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete. Molecular Microbiology, 2016. | pt_BR |
ISSN | 0950-382X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/15272 | |
Sponsorship | National Institutes of Health. Brazilian National Research Council (CNPq). Welch Foundation. | pt_BR |
Language | eng | pt_BR |
Publisher | Wiley | pt_BR |
Rights | open access | pt_BR |
Title | A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete | pt_BR |
Type | Article | pt_BR |
DOI | 10.1111/mmi.13403 | |
Abstract | Leptospira are unique among bacteria based on their helical cell morphology with hook-shaped ends and the presence of periplasmic flagella (PF) with pronounced spontaneous supercoiling. The factors that provoke such supercoiling, as well as the role that PF coiling plays in generating the characteristic hook-end cell morphology and motility, have not been elucidated. We have now identified an abundant protein from the pathogen L. interrogans, exposed on the PF surface, and named it Flagellar-coiling protein A (FcpA). The gene encoding FcpA is highly conserved among Leptospira and was not found in other bacteria. fcpA(-) mutants, obtained from clinical isolates or by allelic exchange, had relatively straight, smaller-diameter PF, and were not able to produce translational motility. These mutants lost their ability to cause disease in the standard hamster model of leptospirosis. Complementation of fcpA restored the wild-type morphology, motility and virulence phenotypes. In summary, we identified a novel Leptospira 36-kDa protein, the main component of the spirochete's PF sheath, and a key determinant of the flagella's coiled structure. FcpA is essential for bacterial translational motility and to enable the spirochete to penetrate the host, traverse tissue barriers, disseminate to cause systemic infection and reach target organs. | pt_BR |
Affilliation | Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Institut Pasteur. Unit of Biology of Spirochetes. Paris, France | pt_BR |
Affilliation | University of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USA | pt_BR |
Affilliation | University of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USA | pt_BR |
Affilliation | Institut Pasteur de Montevideo. Laboratory of Molecular & Structural Microbiology. Montevideo, Uruguay | pt_BR |
Affilliation | University of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USA | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | West Virginia University. Department of Microbiology and ImmunologyMorgantown. WV, USA | pt_BR |
Affilliation | University of Texas Medical School at Houston. Department of Pathology and Laboratory Medicine. Houston, USA / Institute Pasteur. Department of Structural Biology and Chemistry. Paris, France. | pt_BR |
Affilliation | Institut Pasteur. Unit of Biology of Spirochetes. Paris, France | pt_BR |
Affilliation | Yale School of Public Health. Department of Epidemiology of Microbial Disease. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Subject | Leptospira | pt_BR |
Subject | Leptospirosis | pt_BR |
Subject | Spirochete | pt_BR |
Subject | Virulence | pt_BR |
Subject | Flagella | pt_BR |
Subject | Phenotypes | pt_BR |
Subject | Animals | pt_BR |
Subject | Cricetinae | pt_BR |