Author | Paiva, Marcelo Henrique Santos | |
Author | Guedes, Duschinka Ribeiro Duarte | |
Author | Leal, Walter Soares | |
Author | Ayres, Constância Flávia Junqueira | |
Access date | 2017-06-19T19:36:01Z | |
Available date | 2017-06-19T19:36:01Z | |
Document date | 2017 | |
Citation | PAIVA, M. H. S. et al. Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies. Genetics and Molecular Biology, v. 40, n. 3, p. 597–599, 22 maio 2017. | |
ISSN | 1415-4757 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/19419 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies | pt_BR |
Type | Article | en |
DOI | 10.1590/1678-4685-GMB-2016-0312 | pt_BR |
Abstract | Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml). | |
Affilliation | Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | |
Affilliation | Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil. | |
Affilliation | University of California-Davis. Department of Molecular and Cellular Biology. Davis, CA, USA. | |
Affilliation | Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brazil. | |
Subject | ZIKV | |
Subject | RT-PCR | |
Subject | Aedes aegypti | |
Subject | Vector competence | |