Author | Melchior, John T. | |
Author | Walker, Ryan G. | |
Author | Morris, Jamie | |
Author | Jones, Martin K. | |
Author | Segrest, Jere P. | |
Author | Lima, Diogo Borges | |
Author | Carvalho, Paulo Costa | |
Author | Gozzo, Fabio C. | |
Author | Castleberry, Mark | |
Author | Thompson, Thomas B. | |
Author | Davidson, W. Sean | |
Access date | 2017-07-26T16:36:26Z | |
Available date | 2017-07-26T16:36:26Z | |
Document date | 2016 | |
Citation | MELCHIOR, John T. et al. An evaluation of the crystal structure of C-terminal Truncated Apolipoprotein A-I in solution reveals structural dynamics related to Lipid Binding. The Journal of Biological Chemistry, v. 291, n. 10, p. 5439 –5451, March 4, 2016. | pt_BR |
ISSN | 0021-9258 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/20412 | |
Language | eng | pt_BR |
Publisher | American Society for Biochemistry and Molecular Biology | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Lipoproteínas | pt_BR |
Title | An evaluation of the crystal structure of C-Terminal Truncated Apolipoprotein A-I in solution reveals structural dinamics related to Lipid Binding | pt_BR |
Type | Article | pt_BR |
Abstract | Apolipoprotein (apo) A-I mediates many of the anti-atherogenic functions attributed to high density lipoprotein. Unfortunately, efforts toward a high resolution structure of fulllength apoA-I have not been fruitful, although there have been successes with deletion mutants. Recently, a C-terminal truncation (apoA-I185–243) was crystallized as a dimer. The structure showed two helical bundles connected by a long, curved pair of
swapped helical domains. To compare this structure to that existing under solution conditions, we applied small angle x-ray scattering and isotope-assisted chemical cross-linking to apoAI 185–243 in its dimeric and monomeric forms. For the dimer, we found evidence for the shared domains and aspects of the N-terminal
bundles, but not the molecular curvature seen in the crystal. We also found that the N-terminal bundles equilibrate between open and closed states. Interestingly, this movement is one of the transitions proposed during lipid binding. The monomer was consistent with a model in which the long shared helix doubles back onto the helical bundle. Combined with the crystal structure, these data offer an important starting
point to understand the molecular details of high density lipoprotein biogenesis. | pt_BR |
Affilliation | Department of Pathology and Laboratory Medicine. University of Cincinnati. Cincinnati, Ohio, USA. | pt_BR |
Affilliation | Department of Molecular Genetics. Biochemistry and Microbiology. University of Cincinnati. Cincinnati, Ohio, USA. | pt_BR |
Affilliation | Department of Pathology and Laboratory Medicine. University of Cincinnati. Cincinnati, Ohio, USA. | pt_BR |
Affilliation | Department of Medicine and Atherosclerosis Research Unit. University of Alabama at Birmingham. Birmingham, Alabama, USA. | pt_BR |
Affilliation | Department of Medicine and Atherosclerosis Research Unit. University of Alabama at Birmingham. Birmingham, Alabama, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Dalton Mass Spectrometry Laboratory. University of Campinas. São Paulo, SP, Brazil. | pt_BR |
Affilliation | Department of Molecular Genetics. Biochemistry and Microbiology. University of Cincinnati. Cincinnati, Ohio, USA. | pt_BR |
Affilliation | Department of Molecular Genetics. Biochemistry and Microbiology. University of Cincinnati. Cincinnati, Ohio, USA. | pt_BR |
Affilliation | Department of Pathology and Laboratory Medicine. University of Cincinnati. Cincinnati, Ohio, USA. | pt_BR |
Subject | Apolipoproteins | pt_BR |
Subject | Lipid Metabolism | pt_BR |
Subject | Lipoproteins | pt_BR |
Subject in Spanish | Apolipoproteínas | pt_BR |
Subject in Spanish | Metabolismo de los Lípidos | pt_BR |
Subject in Spanish | Lipoproteínas | pt_BR |
DeCS | Apolipoproteínas | pt_BR |
DeCS | Metabolismo dos Lipídeos | pt_BR |