Author | Lyra, Juliana Maria Azevedo de | |
Author | Maruza, Magda | |
Author | Verza, Mirela | |
Author | Carneiro, Maria Madileuza | |
Author | Albuquerque, Maria de Fátima Pessoa Militão de | |
Author | Rossetti, Maria Lúcia | |
Author | Ximenes, Ricardo | |
Author | Braga, Maria Cynthia | |
Author | Lucena-Silva, Norma | |
Access date | 2017-11-17T14:38:38Z | |
Available date | 2017-11-17T14:38:38Z | |
Document date | 2014 | |
Citation | LYRA, uliana Maria Azevedo de et al. Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 109, n. 6, p. 805–813, set. 2014. | pt_BR |
ISSN | 1678-8060 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/23260 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | tuberculose | pt_BR |
Subject in Portuguese | Paucibacilar | pt_BR |
Subject in Portuguese | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
Title | Evaluation of four molecular methods for the diagnosis of tuberculosis in pulmonary and blood samples from immunocompromised patients | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/0074-0276130542 | |
Abstract | The present study analysed the concordance among four different molecular diagnostic methods for tuberculosis (TB) in pulmonary and blood samples from immunocompromised patients. A total of 165 blood and 194 sputum samples were collected from 181 human immunodeficiency virus (HIV)-infected patients with upper respiratory complaints, regardless of suspicious for TB. The samples were submitted for smear microscopy, culture and molecular tests: a laboratory-developed conventional polymerase chain reaction (PCR) and real-time quantitative PCR (qPCR) and the Gen-Probe and Detect-TB Ampligenix kits. The samples were handled blindly by all the technicians involved, from sample processing to results analysis. For sputum, the sensitivity and specificity were 100% and 96.7% for qPCR, 81.8% and 94.5% for Gen-Probe and 100% and 66.3% for Detect-TB, respectively. qPCR presented the best concordance with sputum culture [kappa (k) = 0.864)], followed by Gen-Probe (k = 0.682). For blood samples, qPCR showed 100% sensitivity and 92.3% specificity, with a substantial correlation with sputum culture (k = 0.754) and with the qPCR results obtained from sputum of the corresponding patient (k = 0.630). Conventional PCR demonstrated the worst results for sputa and blood, with a sensitivity of 100% vs. 88.9% and a specificity of 46.3% vs. 32%, respectively. Commercial or laboratory-developed molecular assays can overcome the difficulties in the diagnosis of TB in paucibacillary patients using conventional methods available in most laboratories. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Subject | tuberculosis | pt_BR |
Subject | paucibacillary | pt_BR |
Subject | real-time PCR | pt_BR |
DeCS | Infecções por HIV | pt_BR |
DeCS | sangue | pt_BR |
DeCS | Hospedeiro Imunocomprometido | pt_BR |
DeCS | Mycobacterium tuberculosis | pt_BR |
DeCS | Técnicas de Diagnóstico Molecular | pt_BR |
DeCS | métodos | pt_BR |
DeCS | Escarro | pt_BR |
DeCS | microbiologia | pt_BR |
DeCS | Tuberculose Pulmonar | pt_BR |
DeCS | diagnóstico | pt_BR |
DeCS | Carga Bacteriana | pt_BR |
DeCS | Coinfecção | pt_BR |
DeCS | Primers do DNA | pt_BR |
DeCS | HIV | pt_BR |
DeCS | Pulmão | pt_BR |
DeCS | microbiologia | pt_BR |
DeCS | Mycobacterium tuberculosis | pt_BR |
DeCS | crescimento & desenvolvimento | pt_BR |
DeCS | Kit de Reagentes para Diagnóstico | pt_BR |
DeCS | normas | pt_BR |
DeCS | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
DeCS | métodos | pt_BR |
DeCS | Tuberculose Pulmonar | pt_BR |
DeCS | sangue | pt_BR |
DeCS | Sensibilidade e Especificidade | pt_BR |