Author | Reis, Jordana Grazziela Alves Coelho dos | |
Author | Huang, Jing | |
Author | Tsao, Tiffany | |
Author | Pereira, Felipe V. | |
Author | Funakoshi, Ryota | |
Author | Nakajima, Hiroko | |
Author | Sugiyama, Haruo | |
Author | Tsuji, Moriya | |
Access date | 2018-01-17T17:11:44Z | |
Available date | 2018-01-17T17:11:44Z | |
Document date | 2016 | |
Citation | REIS, Jordana Grazziela Alves Coelho dos et al. Co-administration of alpha-GalCer analog and TLR4 agonist induces robust CD8(+) T-cell responses to PyCS protein and WT-1 antigen and activates memory-like effector NKT cells. Clin Immunol., v. 168, p. 6–15, 2016. | pt_BR |
ISSN | 1521-6616 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/23941 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Adjuvante | pt_BR |
Subject in Portuguese | Glycolipid | pt_BR |
Title | Co-administration of alpha-GalCer analog and TLR4 agonist induces robust CD8(+) T-cell responses to PyCS protein and WT-1 antigen and activates memory-like effector NKT cells. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.clim.2016.04.014 | |
Abstract | In the present study, the combined adjuvant effect of 7DW8-5, a potent α-GalCer-analog, and monophosphoryl lipid A (MPLA), a TLR4 agonist, on the induction of vaccine-induced CD8+ Tcell responses and protective immunity was evaluated. Mice were immunized with peptides corresponding to the CD8+ T-cell epitopes of a malaria antigen, a circumsporozoite protein of Plasmodium yoelii, and a tumor antigen, a Wilms Tumor antigen-1 (WT-1), together with 7DW8-5 and MPLA, as an adjuvant. These immunization regimens were able to induce higher levels of CD8+ T-cell responses and, ultimately, enhanced levels of protection against malaria and tumor challenges compared to the levels induced by immunization with peptides mixed with 7DW8-5 or MPLA alone. Co-administration of 7DW8-5 and MPLA induces activation of memory-like effector natural killer T (NKT) cells, i.e. CD44+CD62L−NKT cells. Our study indicates that 7DW8-5 greatly enhances important synergistic pathways associated to memory immune responses when co-administered with MPLA, thus rendering this combination of adjuvants a novel vaccine adjuvant formulation. | pt_BR |
Affilliation | Rockefeller University. Aaron Diamond AIDS Research Center. New York, NY, USA / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Rockefeller University. Aaron Diamond AIDS Research Center. | pt_BR |
Affilliation | Rockefeller University. Aaron Diamond AIDS Research Center. | pt_BR |
Affilliation | Rockefeller University. Aaron Diamond AIDS Research Center. New York, NY, USA/Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil. | pt_BR |
Affilliation | Rockefeller University. Aaron Diamond AIDS Research Center. New York, NY. | pt_BR |
Affilliation | Osaka University Graduate School of Medicine. Department of Cancer Immunology. Osaka, Japan. | pt_BR |
Affilliation | Osaka University Graduate School of Medicine. Department of Functional Diagnostic Science. Osaka, Japan. | pt_BR |
Affilliation | Rockefeller University. Aaron Diamond AIDS Research Center. | pt_BR |
Subject | Adjuvant | pt_BR |
Subject | Glycolipid | pt_BR |
Subject | NKT cells | pt_BR |
Subject | CD1d | pt_BR |
Subject | TLR4 | pt_BR |
Subject | Malaria vaccine | pt_BR |
Subject | Cancer vaccine | pt_BR |
Subject | WT-1 | pt_BR |
Subject | Circumsporozoite protein | pt_BR |
Subject | Memory-like effector NKT cells | pt_BR |