Author | Hespanhol, Renata | |
Author | Soeiro, Maria de Nazaré C. | |
Author | Meuser, Marcos B. | |
Author | Meirelles, Maria de Nazareth S. L. | |
Author | Côrte-Real, Suzana | |
Access date | 2019-12-10T12:19:09Z | |
Available date | 2019-12-10T12:19:09Z | |
Document date | 2005 | |
Citation | HESPANHOL, Renata C. et al. The Expression of Mannose Receptors in Skin Fibroblast and Their Involvement in Leishmania (L.) amazonensis Invasion. The Journal of Histochemistry & Cytochemistry, v. 53, n.1, p. 35-44, 2005. | pt_BR |
ISSN | 0022-1554 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/37735 | |
Language | eng | pt_BR |
Publisher | SAGE Publications | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Leishmania ( L. ) amazonensis | pt_BR |
Subject in Portuguese | Fibroblastos da pele | pt_BR |
Subject in Portuguese | Receptores de mannose | pt_BR |
Subject in Portuguese | Microscopia eletrônica de transmissão | pt_BR |
Title | The Expression of Mannose Receptors in Skin Fibroblast and Their Involvement in Leishmania (L.) amazonensis Invasion | pt_BR |
Type | Article | pt_BR |
DOI | 10.1369/jhc.4A6384.2005 | |
Abstract | Leishmania
are protozoa that invade mononuclear phagocytes with the involvement
of different ligand-receptor systems, including mannose receptors. Until now,
scant data are available concerning the mechanisms that govern the infection of
Leishmania
in other host cell types such as fibroblasts. Our aim was to analyze the expression of
mannose receptors in primary cultures of skin fibroblasts (SF) further characterizing their
role during the invasion of promastigotes of
Leishmania
(
L
.)
amazonensis
. Both fluorescent,
light, and electron microscopy assays revealed that SF have mannose receptors since they
bound and internalized mannosylated ligands in addition to being positively labeled by
fuc-BSA-FITC probes.
D
-mannose competition assays revealed the participation of mannose receptors
during the parasite association with SF presenting upregulated receptor expression
during the initial steps of the infection. After longer periods of
Leishmania
:fibroblasts contact,
the modulation noted in the host mannose receptors was reverted concomitantly to
the infection control, suggesting that the parasites were required for the alteration maintenance
and providing evidences that the SF may display microbicidal mechanisms to control
the
Leishmania
infection. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Skin fibroblasts | pt_BR |
Subject | Mannose receptors | pt_BR |
Subject | Leishmania ( L. ) amazonensis | pt_BR |
Subject | Transmission electron microscopy | pt_BR |
e-ISSN | 1551-5044 | |