Author | Costa, S. M. | |
Author | Freire, M. S. | |
Author | Alves, A. M. B. | |
Access date | 2020-01-03T15:59:25Z | |
Available date | 2020-01-03T15:59:25Z | |
Document date | 2006 | |
Citation | COSTA, S. M.; FREIRE, M. S.; ALVES, A. M. B. DNA vaccine against the non-structural 1 protein (NSI) of dengue 2 virus. Vaccine, v. 24, p. 4562-4564, 2006. | pt_BR |
ISSN | 0264-410X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/38973 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Vírus da Dengue tipo 2 | pt_BR |
Subject in Portuguese | Vacina de DNA | pt_BR |
Subject in Portuguese | Proteína não estrutural 1 | pt_BR |
Title | DNA vaccine against the non-structural 1 protein (NS1) of dengue 2 virus | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.vaccine.2005.08.022 | |
Abstract | Dengue is one of the most important mosquito-borne viral disease causing dengue fever and/or dengue shock syndrome/haemorrhagic fever. In some reports, the non-structural protein 1 (NS1) has been identified as a promising antigen for the development of vaccines against dengue virus (DENV). Apparently, it can elicit a protective antibody response with complement-fixing activities. In order to investigate the potential of a DNA vaccine based on the NS1 protein against DENV, we used the plasmid pcTPANS1, which contains the secretory signal sequence derived from human tissue plasminogen activator (t-PA) fused to the full length of the DENV-2 NS1 gene. All Balb/c mice intramuscularly inoculated with the pcTPANS1 presented high levels of NS1-specifc antibodies. Vaccinated animals were challenged with intracerebral DENV-2 virus inoculations and a 100% survival was observed. In general, results demonstrate that the pcTPANS1 plasmid is able to induce protection in mice, and then may be used as a vaccination approach against DENV in further assays. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. BioManguinhos. Departamento de Pesquisa e Desenvolvimento. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Dengue 2 virus | pt_BR |
Subject | DNA vaccine | pt_BR |
Subject | Non-structural 1 protein | pt_BR |
e-ISSN | 1873-2518 | |