Author | Cunha, Clarissa F. | |
Author | Nogueira, Raquel Ferraz | |
Author | Costa, Vanessa F. A. | |
Author | Pimentel, Maria Inês F. | |
Author | Chometon, Thaize Q. | |
Author | Lyra, Marcelo R. | |
Author | Schubach, Armando O. | |
Author | Cruz, Alda Maria da | |
Author | Bertho, Álvaro Luiz | |
Access date | 2020-05-07T17:13:49Z | |
Available date | 2020-05-07T17:13:49Z | |
Document date | 2020 | |
Citation | CUNHA, Clarissa F. et al. Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles. Plos One, v. 15, n. 3, p. 1-15, Mar. 2020. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/41152 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmania braziliensis | pt_BR |
Subject in Portuguese | Imunopatogênese cutânea | pt_BR |
Subject in Portuguese | Antígenos | pt_BR |
Subject in Portuguese | Pacientes | pt_BR |
Subject in Portuguese | Citotóxico | pt_BR |
Subject in Portuguese | Perfis de exaustão | pt_BR |
Title | Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.022940 | |
Abstract | The pathogenesis of cutaneous leishmaniasis (CL) caused by Leishmania (Viannia) braziliensis is dictated mainly by the immune-mediated-tissue inflammation developed. The
understanding of the immunological mechanisms that generate tissue damage or resolution
of lesions is the key to the development of effective vaccine protocols and proper therapeutic schemes. It is clear that the specific immune response mediated by T cells is responsible
for the beneficial outcome of the disease, however, the roles of CD4+ T, CD8+ T, NK and
NKT cell subpopulations in immunopathogenesis of CL need to be elucidated. Peripheral
blood cells from patients before, during and after the antimonial therapy, as well as healthy
individuals (HI) were cultured with (LbAgS) or without (NS) L. braziliensis antigens (LbAg).
Afterwards, the frequencies of LbAg-specific-cytotoxic CD8+ T, CD4+ T, NK and
CD3+
CD56+ NKT cells, as well as their activation and exhaustion profiles, were defined by
flow cytometry. We observed higher frequencies of CD8+ T, NK and CD3+
CD56+ NKT cells
and lower frequencies of CD4+ T lymphocytes in LbAgS cell cultures from patients before
treatment. The specific response to LbAg resulted in an expansion of cytotoxic-activated
CD4+ T, CD8+ T, and NK cells, before and during treatment, indicating specificity in the
response by these cells against L. braziliensis. Furthermore, comparing the differences of
frequencies of cytotoxic-activated CD4+
T, CD8+
T, and NK cells, among before and during
treatment patients and HI groups, we conclude that these cell populations are in charge of
immune response elicited by antimonial therapy. Interestingly, we also observed that NK cells were induced by LbAg to an exhaustion profile during all clinical stages of the disease.
The increased antigen-specific activation and cytotoxic activity are in line with the strong
inflammatory response described in this disease, a likely cause of tissue damage. These
findings reinforce the involvement of these distinct cytotoxic-activated cell populations in the
immunopathogenesis of CL, showing a character of specificity in this immune response. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância para Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância para Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância para Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa Médica Interdisciplinar. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Leishmania braziliensis | pt_BR |
Subject | Immunopathogenesis | pt_BR |
Subject | Cuttaneous leishmaniasis | pt_BR |
Subject | Cytotoxic | pt_BR |
Subject | Exhaustion profiles | pt_BR |
Subject | Antigen | pt_BR |