Author | Martins, Gustavo | |
Author | Gogola, Jeferson L. | |
Author | Caetano, Fabio R. | |
Author | Kalinke, Cristiane | |
Author | Jorge, Taíssa R. | |
Author | Santos, Claudia Nunes Duarte dos | |
Author | Bergamini, Márcio F. | |
Author | Marcolino-Junior, Luiz H. | |
Access date | 2020-06-15T17:51:03Z | |
Available date | 2020-06-15T17:51:03Z | |
Document date | 2019 | |
Citation | MARTINS, Gustavo et al. Quick electrochemical immunoassay for hantavirus detection based on biochar platform. Talanta, n. 204, p. 163-171, 2019. | pt_BR |
ISSN | 0039-9140 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/41652 | |
Language | por | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | HCPS | pt_BR |
Subject in Portuguese | Síndrome Cardiopulmonar do Hantavírus | pt_BR |
Subject in Portuguese | Imunossensor Eletroquímico | pt_BR |
Subject in Portuguese | Teste Rápido | pt_BR |
Subject in Portuguese | Biochar | pt_BR |
Title | Quick electrochemical immunoassay for hantavirus detection based on biochar platform | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.talanta.2019.05.101 | |
Abstract | This work describes the first method using biochar (BC) as carbonaceous platform for immunoassay application. BC is a highly functionalized material obtained through biomass pyrolysis under controlled conditions. Due to the highly functionalized surface, covalent binding between BC and biomolecules can be performed by EDC/NHS conjugation. The application of the modified electrode was done with Hantavirus, that are etiologic agents mainly transmitted by wild rodents. Among its pathologies Hantavirus Cardiopulmonary Syndrome (HCPS) arises at Americas, caused by Hantavirus Araucária and reaches 40% lethality. The diagnostic is based on the presence of specific hantavirus nucleoprotein (Np), under viremic condition or IgG2b antibodies (Ab), during first symptoms. The results presented a device sensitivity of 5.28 μA dec-1 and a LOD of 0.14 ng mL-1 to the Np detection, ranging from 5.0 ng mL-1 to 1.0 μg mL-1, the Ab detection works as qualitative type sensor above 200 ng mL-1. Both sensors were evaluated its selectivity and serum samples; selectivity against Gumboro disease, VP2 protein, and antibody IgG2a against Yellow fever disease (YF), respectively. So, the devices here proposed are promising tool suitable for both rodent and human hantavirus clinical surveys. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. | pt_BR |
Subject | Hantavirus Cardiopulmonary Syndrome | pt_BR |
Subject | Electrochemical Immunosensor | pt_BR |
Subject | Quick Test | pt_BR |
Subject | Biomass | pt_BR |
Subject in Spanish | Biomasa | pt_BR |
DeCS | Hantavirus | pt_BR |
DeCS | Biomassa | pt_BR |