Author | Brandão, Adeilton | |
Author | Urmenyi, Turan | |
Author | Rondinelli, Edson | |
Author | Gonzalez, Antonio | |
Author | Miranda, Antonio B. de | |
Author | Degrave, Win | |
Access date | 2020-10-09T14:15:18Z | |
Available date | 2020-10-09T14:15:18Z | |
Document date | 1997 | |
Citation | BRANDÃO, Adeilton et al. Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 6, p. 863-866, Nov./Dec. 1997. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/43902 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Sequenciamento de tags de sequência de sequência expressa | pt_BR |
Subject in Portuguese | Biblioteca cDNA normalizada | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Clone CL Brener | pt_BR |
Subject in Portuguese | Projetos do Genoma do Parasita | pt_BR |
Title | Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02761997000600024 | |
Abstract | Random single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and
forms an integral part of most genome projects, with the objective to identify new genes and targets for
disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome
project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized
library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Instituto de Parasitologia e Biomedicina, Calle Ventanilla. Granada, Spain. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Expressed sequence tags sequencing | pt_BR |
Subject | Normalized cDNA library | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Clone CL Brener | pt_BR |
Subject | Parasite Genome Projects | pt_BR |
e-ISSN | 1678-8060 | |