Author | Saldanha, Maíra Garcia | |
Author | Pagliari, Carla | |
Author | Queiroz, Adriano | |
Author | Machado, Paulo Roberto Lima | |
Author | Carvalho, Lucas | |
Author | Scott, Phillip | |
Author | Carvalho, Edgar Marcelino de | |
Author | Arruda, Sérgio Marcos | |
Access date | 2020-11-11T12:14:58Z | |
Available date | 2020-11-11T12:14:58Z | |
Document date | 2020 | |
Citation | SALDANHA, Maíra Garcia et al. Tissue Damage in Human Cutaneous Leishmaniasis: Correlations Between Inflammatory Cells and Molecule Expression. Frontiers in Cellular and Infection Microbiology, 2020. | pt_BR |
ISSN | 2235-2988 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44372 | |
Sponsorship | National Institutes of Health
(grant AI088650) and Conselho Nacional de Pesquisa Instituto
Nacional de Ciência e Tecnologia—Doenças Tropicais (project
no. 573839/2008-5). The funders had no role in study
design, data collection or analysis, the decision to publish, or
manuscript preparation. | pt_BR |
Language | eng | pt_BR |
Publisher | Frontiers Media | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmaniose Cutânea | pt_BR |
Subject in Portuguese | Inflamação | pt_BR |
Subject in Portuguese | Necrose | pt_BR |
Subject in Portuguese | Amastigotas | pt_BR |
Subject in Portuguese | Tecidos | pt_BR |
Title | Tissue Damage in Human Cutaneous Leishmaniasis: Correlations Between Inflammatory Cells and Molecule Expression | pt_BR |
Type | Article | pt_BR |
DOI | 10.3389/fcimb.2020.00355 | |
Abstract | Cutaneous leishmaniasis (CL) is caused by the bite of the infected sand fly, which
inoculates parasites of Leishmania spp and triggers an immune response. An
exacerbated cutaneous inflammatory response is crucial for controlling parasite burden
but can also promote tissue damage. This study aimed to characterize the populations
of natural killer (NK), CD57+, CD4+, and CD8+ T cells, CD20+ B cells, as well as
CD68+ macrophages, in biopsies of ulcerated CL lesions, and quantify the production of
perforin+, grazyme B+, interleukin 1 beta (IL-1b+) and Tumor Necrosis Factor (TNF-a+
cells). We then correlated these parameters with necrosis, inflammation and the number
of amastigotes. CD4+ T cells were positively correlated to the extent of inflammation,
B cells and IL-1b+ were associated with the extent of necrosis, CD68+ macrophages
and perforin were correlated with the number of amastigotes, and CD57+ NK cells was
correlated to CD68+ macrophages and amastigotes. In sum, the finding suggests that
the production of cytotoxic granules and cytokines by inflammatory cells contributes to
tissue damage in CL lesion | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Universidade São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil. | pt_BR |
Affilliation | University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, PA, United States. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Estadual da Bahia. Departamento de Ciências de Vida. Salvador, BA, Brasil. | pt_BR |
Subject | Cutaneous Leishmaniasis | pt_BR |
Subject | Inflammatory cells | pt_BR |
Subject | Necrosis | pt_BR |
Subject | Amastigotes | pt_BR |
Subject | Tissue damage | pt_BR |