Author | Pereira, Glaucia Vilar | |
Author | Barrios, Leda Castaño | |
Author | Silva, Andrea Alice da | |
Author | Batista, Angelica Martins | |
Author | Pereira, Isabela Resende | |
Author | Moreira, Otacílio Cruz | |
Author | Britto, Constança | |
Author | Santos, Hilton Antônio Mata dos | |
Author | Vieira, Joseli Lannes | |
Access date | 2021-03-30T19:51:52Z | |
Available date | 2021-03-30T19:51:52Z | |
Document date | 2021 | |
Citation | PEREIRA, Glaucia Vilar et al. Memory impairment in chronic experimental Chagas disease: Benznidazole therapy reversed cognitive deficit in association with reduction of parasite load and oxidative stress in the nervous tissue. Plos One, v. 16, n.1, e0244710, 21p, Jan. 2021. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/46469 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Doença de Chagas | pt_BR |
Subject in Portuguese | Terapia de benznidazol | pt_BR |
Subject in Portuguese | Carga de parasitas | pt_BR |
Subject in Portuguese | Estresse oxidativo | pt_BR |
Subject in Portuguese | Tecido nervoso | pt_BR |
Subject in Portuguese | Comprometimento da memória em experimentos crônicos | pt_BR |
Title | Memory impairment in chronic experimental Chagas disease: Benznidazole therapy reversed cognitive deficit in association with reduction of parasite load and oxidative stress in the nervous tissue | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0244710 | |
Abstract | Memory impairment has been associated with chronic Chagas disease (CD), a neglected
tropical disease caused by the protozoan parasite Trypanosoma cruzi. In degenerative diseases,
memory loss has been associated with increased oxidative stress, revealed as
enhanced lipid peroxidation, in the cerebral cortex. Benznidazole (Bz), a trypanocidal drug
efficient to reduce blood parasite load in the acute and chronic phases of infection, showed
controversial effects on heart disease progression, the main clinical manifestation of CD.
Here, we evaluated whether C57BL/6 mice infected with the Colombian type I T. cruzi strain
present memory deficit assessed by (i) the novel object recognition task, (ii) the open field
test and (iii) the aversive shock evoked test, at 120 days post infection (dpi). Next, we tested
the effects of Bz therapy (25mg/Kg/day, for 30 consecutive days) on memory evocation,
and tried to establish a relation between memory loss, parasite load and oxidative stress in
the central nervous system (CNS). At 120 dpi, T. cruzi-infected mice showed memory
impairment, compared with age-matched non-infected controls. Bz therapy (from 120 to
150 dpi) hampered the progression of habituation and aversive memory loss and, moreover,
reversed memory impairment in object recognition. In vehicle-administered infected mice,
neuroinflammation was absent albeit rare perivascular mononuclear cells were found in
meninges and choroid plexus. Bz therapy abrogated the infiltration of the CNS by inflammatory
cells, and reduced parasite load in hippocampus and cerebral cortex. At 120 and 150
dpi, lipid peroxidation was increased in the hippocampus and cortex tissue extracts. Notably,
Bz therapy reduced levels of lipid peroxidation in the cerebral cortex. Therefore, in xperimental chronic T. cruzi infection Bz therapy improved memory loss, in association
with reduction of parasite load and oxidative stress in the CNS, providing a new perspective
to improve the quality of life of Chagas disease patients. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Laboratório Multiusuário de Apoio à Pesquisa em Nefrologia e Ciências Médicas. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Laboratório de Análise e Desenvolvimento de Inibidores Enzimáticos e Laboratório Multiusuário de Análises por RMN. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Chagas disease | pt_BR |
Subject | Benznidazole therapy | pt_BR |
Subject | Parasite load | pt_BR |
Subject | Oxidative stress | pt_BR |
Subject | Nervous tissue | pt_BR |
Subject | Memory impairment in chronic experimental | pt_BR |