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https://www.arca.fiocruz.br/handle/icict/49337
ULTRA-FAST, HIGH THROUGHPUT AND INEXPENSIVE DETECTION OF SARS-COV-2 SEROCONVERSION USING NI2+ MAGNETIC BEADS
Author
Affilliation
Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.
Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.
Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.
Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.
Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
Universidade Federal do Paraná. Programa de Pós-Graduação em Ciências Farmacêuticas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Hospital Erasto Gaertner. Curitiba, PR, Brasil.
Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil.
Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.
Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.
Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.
Organismic Interactions Department. Interfaculty Institute for Microbiology and Infection Medicine. Cluster of Excellence ‘Controlling Microbes to Fight Infections’. Tübingen University, Tübingen, Germany.
Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
Universidade Federal do Paraná. Programa de Pós-Graduação em Ciências Farmacêuticas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Hospital Erasto Gaertner. Curitiba, PR, Brasil.
Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil.
Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
Abstract
To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative
analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni2+ magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.
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