Author | Rêgo, Felipe Dutra | |
Author | Cardoso, Camila D Almeida | |
Author | Moreira, Paulo Otávio Lourenço | |
Author | Nogueira, Paula Monalisa | |
Author | Araújo, Márcio Sobreira Silva | |
Author | Borges, Valéria Matos | |
Author | Laurenti, Márcia D | |
Author | Bartholomeu, Daniella Castanheira | |
Author | Reis, Alexandre Barbosa | |
Author | Monte Neto, Rubens Lima do | |
Author | Soares, Rodrigo Pedro Pinto | |
Access date | 2023-02-01T14:34:09Z | |
Available date | 2023-02-01T14:34:09Z | |
Document date | 2022 | |
Citation | RÊGO, Felipe Dutra et al. Leishmania amazonensis from distinct clinical forms/hosts has polymorphisms in Lipophosphoglycans, displays variations in immunomodulatory properties and, susceptibility to antileishmanial drugs. Cell Biol Int. 2022 Nov;46(11):1947-1958. doi: 10.1002/cbin.11880 | en_US |
ISSN | 1065-6995 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/56812 | |
Language | eng | en_US |
Publisher | John Wiley & Sons Chichester | en_US |
Rights | open access | en_US |
Title | Leishmania amazonensis from distinct clinical forms/hosts has polymorphisms in Lipophosphoglycans, displays variations in immunomodulatory properties and, susceptibility to antileishmanial drugs | en_US |
Type | Article | en_US |
Abstract | Lipophosphoglycan (LPG), the major Leishmania glycoconjugate, induces pro-inflammatory/immunosuppressive innate immune responses. Here, we evaluated functional/biochemical LPG properties from six Leishmania amazonensis strains from different hosts/clinical forms. LPGs from three strains (GV02, BA276, and LV79) had higher pro-inflammatory profiles for most of the mediators, including tumor necrosis factor alpha and interleukin 6. For this reason, glycoconjugates from all strains were biochemically characterized and had polymorphisms in their repeat units. They consisted of three types: type I, repeat units devoid of side chains; type II, containing galactosylated side chains; and type III, containing glucosylated side chains. No relationship was observed between LPG type and the pro-inflammatory properties. Finally, to evaluate the susceptibility against antileishmanial agents, two strains with high (GV02, BA276) and one with low (BA336) pro-inflammatory activity were selected for chemotherapeutic tests in THP-1 cells. All analyzed strains were susceptible to amphotericin B (AmB) but displayed various responses against miltefosine (MIL) and glucantime (GLU). The GV02 strain (canine visceral leishmaniasis) had the highest IC50 for MIL (3.34 μM), whereas diffuse leishmaniasis strains (BA276 and BA336) had a higher IC50 for GLU (6.87-12.19 mM). The highest IC50 against MIL shown by the GV02 strain has an impact on clinical management. Miltefosine is the only drug approved for dog treatment in Brazil. Further studies into drug susceptibility of L. amazonensis strains are warranted, especially in areas where dog infection by this species overlaps with those caused by Leishmania infantum. | en_US |
Affilliation | Biotechnology Applied to Pathogens. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Biotechnology Applied to Pathogens. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Biotechnology Applied to Pathogens. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Biotechnology Applied to Pathogens. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Biotechnology Applied to Pathogens. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Laboratory of Inflammation and Bioarkers. Gonçalo Muniz Institute. Oswaldo Cruz Foundation. Salvador, BA, Brazil. | en_US |
Affilliation | Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, Brazil. | en_US |
Affilliation | Biotechnology Applied to Pathogens. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Biotechnology Applied to Pathogens. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Subject | Leishmania amazonensis | en_US |
Subject | antileishmanial agents | en_US |
Subject | glycoconjugates | en_US |
Subject | innate immunity | en_US |
Subject | lipophosphoglycan | en_US |
Subject | macrophage | en_US |