Author | Ayala, Edward Valencia | |
Author | Cunha, Gisele Rodrigues da | |
Author | Azevedo, Maira Araujo | |
Author | Calderon, Maritza | |
Author | Jimenez, Juan | |
Author | Venuto, Ana Paula | |
Author | Gazzinelli, Ricardo Tostes | |
Author | Lavalle, Raúl Jesus Ynocente | |
Author | Riva, Angela Giovana Vidal | |
Author | Hincapie, Robert | |
Author | Finn, M. G. | |
Author | Marques, Alexandre F. | |
Access date | 2023-05-25T18:29:10Z | |
Available date | 2023-05-25T18:29:10Z | |
Document date | 2020 | |
Citation | AYALA, Edward Valencia. C57BL/6 α-1,3-Galactosyltransferase Knockout Mouse as an Animal Model for Experimental Chagas Disease. ACS Infect. Dis., v. 6, n. 7, p. 1807–18150, 2020. doi.org/10.1021/acsinfecdis.0c00061 | en_US |
ISSN | 2373-8227 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/58728 | |
Language | eng | en_US |
Publisher | American Chemical Society | en_US |
Rights | restricted access | en_US |
Title | C57BL/6 alpha-1,3-Galactosyltransferase Knockout Mouse as an Animal Model for Experimental Chagas Disease | en_US |
Type | Article | en_US |
DOI | 10.1021/acsinfecdis.0c00061 | |
Abstract | The leading animal model of experimental Chagas disease, the mouse, plays a significant role in studies for vaccine development, diagnosis, and human therapies. Humans, along with Old World primates, alone among mammals, cannot make the terminal carbohydrate linkage of the alpha-Gal trisaccharide. It has been established that the anti-alpha-Gal immune response is likely to be a critical factor for protection against Trypanosoma cruzi (T. cruzi) infection in humans. However, the mice customarily employed for the study of T. cruzi infection naturally express the alpha-Gal epitope and therefore do not produce anti-alpha-Gal antibodies. Here, we used the C57BL/6 alpha-1,3-galactosyltransferase knockout (alpha-GaIT-KO) mouse, which does not express the alpha-Gal epitope as a model for experimental Chagas disease. We found the anti-alpha-Gal IgG antibody response to an increase in alpha-GaIT-KO mice infected with Arequipa and Colombiana strains of T. cruzi, leading to fewer parasite nests, lower parasitemia, and an increase of INF-gamma, TNF-alpha, and IL-12 cytokines in the heart of alpha-GaIT-KO mice compared with alpha-GaIT-WT mice on days 60 and 120 postinfection. We therefore agree that the C57BL/6 alpha-GaIT-KO mouse represents a useful model for initial testing of therapeutic and immunological approaches against different strains of T. cruzi. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Imuno-Proteômica e Biologia de Parasitos. Belo Horizonte, MG, Brazil/Instituto de Investigación. Centro de Investigación en Inmunología e Infectología. Facultad de Medicina Humana. Universidad de San Martin de Porres. Lima, Perú | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Imuno-Proteômica e Biologia de Parasitos. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Imuno-Proteômica e Biologia de Parasitos. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Laboratorio de Investigación en Enfermedades Infecciosas and Laboratorio de Biología Molecular. Facultad de Ciencias y Filosofía. Universidad Peruana Cayetano Heredia. Lima, Perú | en_US |
Affilliation | Laboratorio de Parasitología en Fauna Silvestre y Zoonosis. Facultad de Ciencias Biológicas. Universidad Nacional Mayor de San Marcos. Lima, Perú | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Imuno-Proteômica e Biologia de Parasitos. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Plataforma de Medicina Translacional. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Laboratorio de Parasitología en Fauna Silvestre y Zoonosis. Facultad de Ciencias Biológicas. Universidad Nacional Mayor de San Marcos. Lima, Perú | en_US |
Affilliation | Instituto de Investigación. Centro de Investigación en Inmunología e Infectología. Facultad de Medicina Humana. Universidad de San Martin de Porres. Lima, Perú/Laboratorio de Investigación en Enfermedades Infecciosas and Laboratorio de Biología Molecular. Facultad de Ciencias y Filosofía. Universidad Peruana Cayetano Heredia. Lima, Perú | en_US |
Affilliation | School of Chemistry and Biochemistry. School of Biological Sciences. Georgia Institute of Technology. Atlanta, Georgia, United States | en_US |
Affilliation | School of Chemistry and Biochemistry. School of Biological Sciences. Georgia Institute of Technology. Atlanta, Georgia, United States | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Imuno-Proteômica e Biologia de Parasitos. Belo Horizonte, MG, Brazil | en_US |
Subject | Chagas disease | en_US |
Subject | α-Gal mouse model | en_US |
Subject | Trypanosoma cruzi | en_US |
Subject | α-Gal antibodies | en_US |
Subject | α-Gal knockout mouse | en_US |
Embargo date | 2099-12-31 | |