Author | Bentes, Amaury dos Santos | |
Author | Wanke, Bodo | |
Author | Lazéra, Márcia dos Santos | |
Author | Freire, Ana Karla Lima | |
Author | Silva Júnior, Roberto Moreira da | |
Author | Rocha, Diego Fernando Silva | |
Author | Pinheiro, Silviane Bezerra | |
Author | Zelski, Steven Edward | |
Author | Matsuura, Ani Beatriz Jackisch | |
Author | Rocha, Liliane Coelho da | |
Author | Souza, Erica Simplício de | |
Author | Souza, João Vicente Braga de | |
Access date | 2024-01-06T00:11:09Z | |
Available date | 2024-01-06T00:11:09Z | |
Document date | 2019 | |
Citation | BENTES, Amaury dos Santos et al. Cryptococcus gattii VGII isolated from native forest and river in Northern Brazil. Brazilian Journal of Microbiology, v. 50, p. 495–500, 2019. | en_US |
ISSN | 1517-8382 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/62087 | |
Language | eng | en_US |
Publisher | Sociedade Brasileira de Microbiologia | en_US |
Rights | open access | en_US |
Title | Cryptococcus gattii VGII isolated from native forest and river in Northern Brazil | en_US |
Type | Article | en_US |
DOI | 10.1007/s42770-019-00066-6 | |
Abstract | Background Cryptococcosis is a global invasive mycosis associated with significant morbidity and mortality. In the northern region of Brazil, this disease is caused by Cryptococcus neoformans genotype VNI and Cryptococcus gattii genotype VGII. However, few environmental studies have been conducted in this large tropical area.
Aims This study was performed to isolate, genotype, and determine the frequency of cryptococcal agents in environmental samples near Manaus, Amazonas, Brazil.
Methods A total of 970 environmental samples (290 from soil, 290 from decaying plants, 5 from insects, 280 from the Negro river, and 105 from small streams within the city of Manaus) were collected and plated on Niger seed agar. In addition, 20 subcultures obtained from each positive sample were analyzed by PCR-RFLP (URA5) and PCR for genotyping and determination of mating type. Results Six samples were positive for isolates from the C. gattii species complex. Of those, three samples were from Adolpho Ducke Forest Reserve and three were from the Negro river. All isolates were C. gattii genotype VGII (mating type MATα). Conclusion Genotype VGII proved to be the most important genotype found in the environmental samples. The genotype VGII has been described as one of the most virulent and less susceptible to antifungals and responsible for important outbreaks. This is the first study to demonstrate isolation of C. gattii (VGII) from the Negro river. | en_US |
Affilliation | Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisas Clínicas Evandro Chagas. Laboratório de Micologia Médico. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisas Clínicas Evandro Chagas. Laboratório de Micologia Médico. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil. | en_US |
Affilliation | Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil. | en_US |
Affilliation | Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil. | en_US |
Affilliation | Instituto Nacional de Pesquisas da Amazônia. Laboratório de Micologia Médica. Manaus, AM, Brasil. | en_US |
Affilliation | Miami Univeristy. Middletown, OH, USA. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil. | en_US |
Affilliation | Universidade do Estado do Amazonas. Manaus, AM, Brasil. | en_US |
Affilliation | Universidade do Estado do Amazonas. Manaus, AM, Brasil. | en_US |
Affilliation | Instituto Nacional de Pesquisas da Amazônia. Laboratório de Micologia Médica. Manaus, AM, Brasil. | en_US |
Subject | Amazon Region | en_US |
Subject | Cryptococcus | en_US |
Subject | Genotyping | en_US |
Subject | Isolation | en_US |
Subject | M13 PCR fingerprinting | en_US |
Subject | PCR-RFLP of URA5 | en_US |
e-ISSN | 1678-4405 | |