Author | Pereira, Felicidade Mota | |
Author | Zarife, Maria Alice Sant’Anna | |
Author | Reis, Eliana Almeida Gomes | |
Author | Reis, Mitermayer Galvão dos | |
Access date | 2014-06-04T19:18:47Z | |
Available date | 2014-06-04T19:18:47Z | |
Document date | 2014 | |
Citation | PEREIRA, F. M. et al. Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. Revista da Sociedade Brasileira de Medicina Tropical, v. 47, n. 1, p. 12-17, 2014. | pt_BR |
ISSN | 1678-9849 | |
URI | https://www.arca.fiocruz.br/handle/icict/7795 | |
Language | eng | pt_BR |
Publisher | Sociedade Brasileira de Medicina Tropical | pt_BR |
Rights | open access | pt_BR |
Title | Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA) in blood donors. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/0037-8682-0222-2013 | |
Abstract | INTRODUCTION: Hepatitis C virus (HCV) infection is diagnosed by the presence of antibodies and is supplemented by confirmatory testing methods, such as recombinant immunoblot assay (RIBA) and HCV-RNA detection. This study aimed to evaluate the efficacy of RIBA testing to diagnose HCV infection in blood donors positive for anti-HCV antibodies. METHODS: A total of 102 subjects positive for anti-HCV determined by enzyme-linked immunosorbent assay (ELISA) at the Hematology and Hemotherapy Foundation of Bahia (HEMOBA) were later assessed with new samples using the Abbott Architect anti-HCV test (Abbott Diagnostics, Wiesbaden, Germany), the RIBA III test (Chiron RIBA HCV 3.0 SIA, Chiron Corp., Emeryville, CA, USA), the polymerase chain reaction (PCR; COBAS® AMPLICOR HCV Roche Diagnostics Corp., Indianapolis, IN, USA) and line probe assay (LiPA - Siemens, Tarrytown, NY, USA) genotyping for HCV diagnosis. RESULTS: Of these new samples, 38.2% (39/102) were positive, 57.8% (59/102) were negative and 3.9% (4/102) were indeterminate for anti-HCV; HCV-RNA was detected in 22.5% (23/102) of the samples. RIBA results were positive in 58.1% (25/43), negative in 9.3% (4/43) and indeterminate in 32.6% (14/43) of the samples. The prevailing genotypes were 1 (78.3%, 18/23), 3 (17.4%, 4/23) and 2 (4.3%, 1/23). All 14 samples with indeterminate RIBA results had undetectable viral loads (detection limit ≤50 IU/mL). Of these samples, 71.4% (10/14) were reevaluated six months later. Eighty percent (8/10) of these samples remained indeterminate by RIBA, and 20% (2/10) were negative. CONCLUSIONS: In this study, individuals with indeterminate RIBA results had no detectable HCV-RNA. | pt_BR |
Affilliation | Laboratório Central de Saúde Pública do Estado da Bahia. Secretaria da Saúde. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil | pt_BR |
Affilliation | Laboratório Central de Saúde Pública do Estado da Bahia. Secretaria da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Faculdade de Medicina da Bahia. Universidade Federal da Bahia. Salvador, BA, Brasil / Escola Baiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
Subject | Blood donors | pt_BR |
Subject | Hepatitis C virus | pt_BR |
Subject | Anti-HCV | pt_BR |
Subject | RIBA. | pt_BR |
Subject | HCV-RNA | pt_BR |
DeCS | Doadores de Sangue | pt_BR |
DeCS | Hepacivirus/genética | pt_BR |
DeCS | Anticorpos Anti-Hepatite C/sangue | pt_BR |
DeCS | Hepatite C/diagnóstico | pt_BR |
DeCS | RNA Viral/sangue | pt_BR |
DeCS | Ensaio de Imunoadsorção Enzimática | pt_BR |
DeCS | Adulto | pt_BR |
DeCS | Feminino | pt_BR |
DeCS | Genótipo | pt_BR |
DeCS | Hepacivirus/imunologia | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Immunoblotting | pt_BR |
DeCS | Masculino | pt_BR |
DeCS | Reação em Cadeia da Polimerase | pt_BR |
DeCS | Proteínas Recombinantes/uso diagnóstico | pt_BR |
DeCS | Carga Viral | pt_BR |