Author | Teixeira, Clarissa Romero | |
Author | Gomes, Regis Bernardo Brandim | |
Author | Collin, Nicolas | |
Author | Reynoso, David | |
Author | Jochim, Ryan | |
Author | Oliveira, Luiz Fabiano Borges | |
Author | Seitz, Amy | |
Author | Elnaiem, Dia-Eldin A | |
Author | Caldas, Arlene de Jesus Mendes | |
Author | Souza, Ana Paula Almeida de | |
Author | Brodskyn, Claudia Ida | |
Author | Oliveira, Camila Indiani de | |
Author | Mendonça, Ivete Lopes de | |
Author | Costa, Carlos Henrique Nery | |
Author | Volf, Petr | |
Author | Barral, Aldina Maria Prado | |
Author | Kamhawi, Shaden | |
Author | Valenzuela, Jesus G | |
Access date | 2014-09-16T12:11:13Z | |
Available date | 2014-09-16T12:11:13Z | |
Document date | 2010 | |
Citation | TEIXEIRA, C. et al. Discovery of markers of exposure specific to bites of Lutzomyia longipalpis, the vector of Leishmania infantum chagasi in Latin America. PLoS Neglected Tropical Diseases, v. 4, n. 3, p. e638, 2010. | pt_BR |
ISSN | 1935-2735 | |
URI | https://www.arca.fiocruz.br/handle/icict/8384 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Title | Discovery of markers of exposure specific to bites of Lutzomyia longipalpis, the vector of Leishmania infantum chagasi in Latin America | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pntd.0000638 | |
Abstract | BACKGROUND: Sand flies deliver Leishmania parasites to a host alongside salivary molecules that affect infection outcomes. Though some proteins are immunogenic and have potential as markers of vector exposure, their identity and vector specificity remain elusive. METHODOLOGY/PRINCIPAL FINDINGS: We screened human, dog, and fox sera from endemic areas of visceral leishmaniasis to identify potential markers of specific exposure to saliva of Lutzomyia longipalpis. Human and dog sera were further tested against additional sand fly species. Recombinant proteins of nine transcripts encoding secreted salivary molecules of Lu. longipalpis were produced, purified, and tested for antigenicity and specificity. Use of recombinant proteins corresponding to immunogenic molecules in Lu. longipalpis saliva identified LJM17 and LJM11 as potential markers of exposure. LJM17 was recognized by human, dog, and fox sera; LJM11 by humans and dogs. Notably, LJM17 and LJM11 were specifically recognized by humans exposed to Lu. longipalpis but not by individuals exposed to Lu. intermedia. CONCLUSIONS/SIGNIFICANCE: Salivary recombinant proteins are of value as markers of vector exposure. In humans, LJM17 and LJM11 emerged as potential markers of specific exposure to Lu. longipalpis, the vector of Leishmania infantum chagasi in Latin America. In dogs, LJM17, LJM11, LJL13, LJL23, and LJL143 emerged as potential markers of sand fly exposure. Testing these recombinant proteins in large scale studies will validate their usefulness as specific markers of Lu. longipalpis exposure in humans and of sand fly exposure in dogs. | pt_BR |
Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America | |
Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America | |
Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America | |
Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America | |
Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America | |
Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America | |
Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America | |
Affilliation | University of Maryland Eastern Shore. Department of Natural Sciences. Princess Anne. Maryland, United States of America | |
Affilliation | Universidade Federal do Maranhão. São Luis, MA, Brasil | |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil | |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil | |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil | |
Affilliation | Universidade Federal do Piaui. Instituto de Doenças Tropicais Natan Portella. Laboratório de Leishmanioses. Teresina, PI, Brasil | |
Affilliation | Universidade Federal do Piaui. Instituto de Doenças Tropicais Natan Portella. Laboratório de Leishmanioses. Teresina, PI, Brasil | |
Affilliation | Charles University in Prague. Faculty of Science. Department of Parasitology. Prague, Czech Republic | |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil | |
Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America | |
Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America | |
DeCS | Vetores de Doenças | pt_BR |
DeCS | Mordeduras e Picadas de Insetos/imunologia | pt_BR |
DeCS | Proteínas de Insetos/imunologia | pt_BR |
DeCS | Psychodidae/imunologia | pt_BR |
DeCS | Saliva/imunologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Anticorpos/sangue | pt_BR |
DeCS | Cães | pt_BR |
DeCS | Raposas | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Proteínas de Insetos/uso diagnóstico | pt_BR |
DeCS | Proteínas de Insetos/genética | pt_BR |
DeCS | América Latina | pt_BR |
DeCS | Proteínas Recombinantes/uso diagnóstico | pt_BR |
DeCS | Proteínas Recombinantes/genética | pt_BR |
DeCS | Proteínas Recombinantes/imunologia | pt_BR |
DeCS | Sensibilidade e Especificidade | pt_BR |