Author | Machado, Anderson Silva | |
Author | Carneiro, Ana Carolina Aguiar Vasconcelos | |
Author | Béla, Samantha Ribeiro | |
Author | Andrade, Gláucia Manzan Queiroz | |
Author | Santos, Daniel Vitor Vasconcelos | |
Author | Januário, José Nélio | |
Author | Reis, Jordana Grazziela Alves Coelho dos | |
Author | Ferro, Eloisa Amália Vieira | |
Author | Carvalho, Andréa Teixeira | |
Author | Vitor, Ricardo Wagner Almeida | |
Author | Martins Filho, Olindo Assis | |
Access date | 2015-03-23T19:05:59Z | |
Available date | 2015-03-23T19:05:59Z | |
Document date | 2014 | |
Citation | MACHADO, Anderson Silva et al. Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. Mediators Inflamm, v. 2014, p. 910621, 2014 | pt_BR |
ISSN | 0962-9351 | |
URI | https://www.arca.fiocruz.br/handle/icict/9812 | |
Language | eng | pt_BR |
Publisher | Hindawi Pub. Corp | pt_BR |
Rights | open access | pt_BR |
Title | Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1155/2014/910621. | |
Abstract | Toxoplasma gondii is the main infectious cause of human posterior retinochoroiditis, the most frequent clinical manifestation of congenital toxoplasmosis. This investigation was performed after neonatal screening to identify biomarkers of immunity associated with immunopathological features of the disease by flow cytometry. The study included infected infants without NRL and with retinochoroidal lesions (ARL, ACRL, and CRL) as well as noninfected individuals (NI). Our data demonstrated that leukocytosis, with increased monocytes and lymphocytes, was a relevant hematological biomarker of ARL. Immunophenotypic analysis also revealed expansion of CD14(+)CD16(+)HLA-DR(high) monocytes and CD56(dim) cytotoxic NK-cells in ARL. Moreover, augmented TCRγ δ (+) and CD8(+) T-cell counts were apparently good biomarkers of morbidity. Biomarker network analysis revealed that complex and intricated networks underscored the negative correlation of monocytes with NK- and B-cells in NRL. The remarkable lack of connections involving B-cells and a relevant shift of NK-cell connections from B-cells toward T-cells observed in ARL were outstanding. A tightly connected biomarker network was observed in CRL, with relevant connections of NK- and CD8(+) T-cells with a broad range of cell subsets. Our findings add novel elements to the current knowledge on the innate and adaptive immune responses in congenital toxoplasmosis | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia.Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia.Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Pediatria. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Oftalmologia e Otorrinolaringologia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Nucleo de Acoes e Pesquisa em Apoio Diagnostico. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Uberlandia. Uberlandia, MG, Brazil | pt_BR |
Affilliation | Fundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia.Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, Brazil /Universidade Federal de Minas Gerais. Congenital Toxoplasmosis Brazilian Group | pt_BR |
Subject | Toxoplasma gondii | pt_BR |
Subject | Congenital Toxoplasmosis | pt_BR |