Author | Stoco, Patrícia Hermes | |
Author | Wagner, Glauber | |
Author | Talavera-Lopez, Carlos | |
Author | Gerber, Alexandra | |
Author | Zaha, Arnaldo | |
Author | Thompson, Claudia Elizabeth | |
Author | Bartholomeu, Daniella Castanheira | |
Author | Luckemeyer, Debora Denardin | |
Author | Bahia, Diana | |
Author | Loreto, Elgion | |
Author | Prestes, Elisa Beatriz | |
Author | Lima, Fabio Mitsuo | |
Author | Rodrigues Luiz, Gabriela | |
Author | Vallejo, Gustavo Adolfo | |
Author | Silveira Filho, Jose Franco da | |
Author | Schenkman, Sergio | |
Author | Monteiro, Karina Mariante | |
Author | Tyler, Kevin Morris | |
Author | Almeida, Luiz Gonzaga Paula de | |
Author | Ortiz, Mauro Freitas | |
Author | Chiurillo, Miguel Angel | |
Author | Moraes, Milene Hoehr de | |
Author | Cunha, Oberdan de Lima | |
Author | Mendonça Neto, Rondon | |
Author | Silva, Rosane | |
Author | Teixeira, Santuza Maria Ribeiro | |
Author | Murta, Silvane Maria Fonseca | |
Author | Sincero, Thais Cristine Marques | |
Author | Mendes, Tiago Antonio de Oliveira | |
Author | Urmenyi, Turan Peter | |
Author | Silva, Viviane Grazielle | |
Author | Rocha, Wanderson Duarte Da | |
Author | Andersson, Bjorn | |
Author | Romanha, Alvaro Jose | |
Author | Steindel, Mario | |
Author | Vasconcelos, Ana Tereza Ribeiro de | |
Author | Grisard, Edmundo Carlos | |
Access date | 2015-03-27T12:45:23Z | |
Available date | 2015-03-27T12:45:23Z | |
Document date | 2015 | |
Citation | STOCO, Patrıcia Hermes et al. Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli. PLoS Negl Trop Dis, v. 8, n. 9, p. e3176. | pt_BR |
ISSN | 1553-7366 | |
URI | https://www.arca.fiocruz.br/handle/icict/9850 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Title | Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli | pt_BR |
Type | Article | pt_BR |
DOI | Public Library of Science | |
Abstract | BACKGROUND:Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts.
METHODOLOGY/PRINCIPAL FINDINGS:The T. rangeli haploid genome is ∼ 24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heat-shock proteins.
CONCLUSIONS/SIGNIFICANCE: Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets. | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil/Universidade do Oeste de Santa Catarina. Joaçaba, SC, Brazil | pt_BR |
Affilliation | Karolinska Institutet. Science for Life Laboratory. Department of Cell and Molecular Biology. Stockholm, Sweden | pt_BR |
Affilliation | Laboratorio Nacional de Computação Cientıfica. Petropolis, Rio de Janeiro, Brazil | pt_BR |
Affilliation | Universidade Federal do Rio Grande do Sul. Porto Alegre, RG, Brazil | pt_BR |
Affilliation | Laboratorio Nacional de Computação Cientıfica. Petropolis, Rio de Janeiro, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil/Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo, SP, Brazil | pt_BR |
Affilliation | Universidade Federal de Santa Maria. Santa Maria, RG, Brazil | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo, SP, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidad del Tolima. Ibague, Colombia | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo, SP, Brazil | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo, SP, Brazil | pt_BR |
Affilliation | Universidade Federal do Rio Grande do Sul. Porto Alegre, RG, Brazil | pt_BR |
Affilliation | Universidad Centroccidental Lisandro Alvarado. Barquisimeto, Venezuela | pt_BR |
Affilliation | Laboratorio Nacional de Computação Cientıfica. Petropolis, Rio de Janeiro, Brazil | pt_BR |
Affilliation | Universidade Federal do Rio Grande do Sul. Porto Alegre, RG, Brazil | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo, SP, Brazil/ Universidad Centroccidental Lisandro Alvarado. Barquisimeto, Venezuela | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil | pt_BR |
Affilliation | Laboratorio Nacional de Computação Cientıfica. Petropolis, RJ, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal do Parana. Curitiba, PR, Brazil | pt_BR |
Affilliation | Karolinska Institutet. Science for Life Laboratory. Department of Cell and Molecular Biology. Stockholm, Sweden | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil | pt_BR |
Affilliation | Karolinska Institutet. Science for Life Laboratory. Department of Cell and Molecular Biology. Stockholm, Sweden | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Florianopolis, SC, Brazil | pt_BR |
Subject | Trypanosoma rangeli | pt_BR |
Subject | Chagas disease | pt_BR |