| Author | Araújo, Catarina A. C | |
| Author | Waniek, Peter J | |
| Author | Jansen, Ana Maria | |
| Access date | 2015-04-22T14:23:07Z | |
| Available date | 2015-04-22T14:23:07Z | |
| Document date | 2014 | |
| Citation | ARAÚJO, Catarina A; WANIEK, Peter J.; JANSEN, Ana M. TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus. Parasites & Vectors, v.7, n.94, 8p, 2014. | pt_BR |
| ISSN | 1756-3305 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10093 | |
| Description | We thank Prof. J. Jurberg (Laboratório Nacional e Internacional de Referência
em Taxonomia de Triatomíneos, Fiocruz, Rio de Janeiro) for providing the
insects and Prof. Dr. V. Bongertz (Laboratório de AIDS e Imunologia Molecular,
Fiocruz, Rio de Janeiro) for the English corrections. This work was supported by
Fundação de Amparo à Pesquisa no Estado do Rio de Janeiro (FAPERJ: E-26/
100.456/2007), Conselho Nacional de Desenvolvimento Científico e Tecnológico
(CNPq: Edital Universal 472276/2006-9; PDJ: 152069/2006-2) and Fundação
Oswaldo Cruz (FIOCRUZ). CACA was a CNPq Research Fellow (151187/2009-6)
and PJW was a FAPERJ Research Fellow (E-26/152.913/2005). | pt_BR |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma | pt_BR |
| Subject in Portuguese | Trato Gastrointestinal | pt_BR |
| Subject in Portuguese | Doença de Chagas | pt_BR |
| Subject in Portuguese | Rhodnius | pt_BR |
| Title | TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus | pt_BR |
| Type | Article | |
| DOI | 10.1186/1756-3305-7-94 | pt_BR |
| Abstract | Background: Rhodnius prolixus is an obligate haematophagous insect and one of the most important vectors of
Trypanosoma cruzi, the causative agent of Chagas disease in the Americas. T. cruzi is a highly variable parasite which
is not transmitted in the same efficiency by the different triatomine vectors. Because different T. cruzi genotypes are
aetiopathologically divergent, further elucidation of the transmission abilities of different Chagas disease vectors is
extremely important.
Findings: In the present study, the growth behaviour of two T. cruzi isolates, MDID/BR/1993/C45 (TcI) and TBRA/BR/
1999/JCA3 (TcII), sharing the same microhabitat (intestinal tract) in single and mixed infections, was examined. The
distribution patterns and parasite population densities were evaluated at 7, 14 and 21 days after feeding (daf) by
quantification of parasites using Neubauer haemocytometric measurements and mini-exon PCR to identify TcI and
TcII subpopulations. Parasitic colonization in the small intestine was more successful in the mixed infection model
than the single infection models at 21 daf. In the rectal lumen and wall, the growth behaviour of the mixed infection
was similar to that of the TcI group, although the total parasite number was lower. In the TcII group, no metacyclic
trypomastigote forms were found. PCR analysis of the contents of each dissected region showed different genotype
fractions in the mixed infection model, in which TcI seemed to be the predominant isolate.
Conclusion: The different growth behaviour of the TcI and TcII isolates in single and mixed infection models
demonstrated that possibly an intraspecific factor modulates parasitic development in the intestine of R. prolixus. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Tcl | pt_BR |
| Subject | Tdl | pt_BR |
| Subject | Mixed Infections | pt_BR |
| Subject | Rhodnius prolixus | pt_BR |
| Subject | Digestive tract | pt_BR |
| Subject | Chagas Disease | pt_BR |
| Subject in Spanish | Tracto Gastrointestinal | pt_BR |
| Subject in Spanish | Trypanosoma | pt_BR |
| Subject in Spanish | Enfermedad de Chagas | pt_BR |
| Subject in Spanish | Rhodnius | pt_BR |
| DeCS | Rhodnius | pt_BR |
| DeCS | Trato Gastrointestinal | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
