| Author | Silva, Elisabeth Martins da | |
| Author | Guillermo, Landi Veivi Costilla | |
| Author | Gomes, Flávia Lima Ribeiro | |
| Author | Meis, Juliana De | |
| Author | Nunes, Marise Pinheiro | |
| Author | Senra, Juliana Fraga Vasconcelos | |
| Author | Soares, Milena Botelho Pereira | |
| Author | Reis, George Alexandre dos | |
| Author | Lopes, Marcela de Freitas | |
| Access date | 2015-04-22T18:34:19Z | |
| Available date | 2015-04-22T18:34:19Z | |
| Document date | 2007 | |
| Citation | SILVA, E. M. et al. Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection. European Joural of Immunology, v. 37, n. 3, p. 738-746, 2007. | pt_BR |
| ISSN | 0014-2980 | |
| URI | https://www.arca.fiocruz.br/handle/icict/10113 | |
| Language | eng | pt_BR |
| Publisher | Wiley-VCH Verlag GmbH & Co | pt_BR |
| Rights | open access | pt_BR |
| Title | Caspase inhibition reduces lymphocyte apoptosis and improves host immune responses to Trypanosoma cruzi infection | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1002/eji.200636790 | |
| Abstract | In experimental Chagas' disease, lymphocytes from mice infected with Trypanosoma cruzi show increased apoptosis in vivo and in vitro. Treatment with a pan-caspase blocker peptide inhibited expression of the active form of effector caspase-3 in vitro and rescued both B and T cells from cell death. Injection of the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethyl ketone, but not a control peptide, reduced parasitemia and lymphocyte apoptosis in T. cruzi-infected mice. Moreover, treatment with caspase inhibitor throughout acute infection increased the absolute numbers of B and T cells in the spleen and lymph nodes, without affecting cell infiltrates in the heart. Following treatment, we found increased accumulation of memory/activated CD4 and CD8 T cells, and secretion of IFN-gamma by splenocytes stimulated with T. cruzi antigens. Caspase inhibition in the course of infection reduced the intracellular load of parasites in peritoneal macrophages, and increased the production of TNF-alpha and nitric oxide upon activation in vitro. Our results indicate that inhibition of caspases with a pan-caspase blocker peptide improves protective type-1 immune responses to T. cruzi infection. We suggest that mechanisms of apoptosis are potential therapeutic targets in Chagas' disease. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Apoptosis | pt_BR |
| Subject | Caspases | pt_BR |
| Subject | Infection | pt_BR |
| Subject | Lymphocyte | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| DeCS | Apoptose/imunologia | pt_BR |
| DeCS | Inibidores de Caspase | pt_BR |
| DeCS | Doença de Chagas/imunologia | pt_BR |
| DeCS | Doença de Chagas/patologia | pt_BR |
| DeCS | Linfócitos/enzimologia | pt_BR |
| DeCS | Trypanosoma cruzi/imunologia | pt_BR |
| DeCS | Clorometilcetonas de Aminoácidos/farmacologia | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Apoptose/efeitos de drogas | pt_BR |
| DeCS | Doença de Chagas/quimioterapia | pt_BR |
| DeCS | Linfócitos/efeitos de drogas | pt_BR |
| DeCS | Masculino | pt_BR |
| DeCS | Camundongos | pt_BR |
| DeCS | Camundongos Endogâmicos BALB C | pt_BR |
